ICEberg
I. Information of ICE
ICEberg ID987
Name ICEMhThis ICE is derived from experimental literature
FamilyUnclassified
OrganismMannheimia haemolytica 42548
Size (bp)92345
GC content [Genome] (%)40.18[41.05]
Insertion sitetRNALeu (DR:5'-GATTTTGAATC-3')
FunctionResistance to streptomycin, kanamycin/neomycin (aphA1), tetracycline [tetR-tet(H)], sulphonamides (sul2).
Species that ICE can be transferred toPasteurella multocida
Nucleotide SequenceCP005383 (partial ICE sequence)
Putative oriT region -
Putative relaxase coordinates: 2136369..2138348; Locus tag: MHH_c22640;  Family:  MOBH


II. ICE interaction with IME/CIME/

The interaction information of ICEMh1 is not available.



The graph information of ICEMh1 components from CP005383
Incomplete gene list of ICEMh1 from CP005383
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1MHH_c225102125864..2126262 [-], 399conjugative transfer region lipoprotein
2MHH_c225202126275..2126739 [-], 465integrating conjugative element protein
3MHH_c225302126825..2127352 [-], 528hypothetical protein
4MHH_c225402127555..2128094 [+], 540acyl-coenzyme A:6-aminopenicillanic acid acyl-transferase
5aphA12128248..2129063 [-], 816aminoglycoside 3'-phosphotransferase protein AphAAR 
6strB2129399..2130229 [-], 831aminoglycoside 6-phosphotransferase protein StrBAR 
7strA2130235..2131095 [-], 861aminoglycoside 3''-phosphotransferase protein StrAAR 
8sulII2131207..2132022 [-], 816dihydropteroate synthase Sul2AR 
9MHH_c225902132216..2132404 [-], 189putative transcriptional regulator
10MHH_c226002132863..2133705 [+], 843putative Mhp-like hydrolase
11MHH_c226302135449..2136213 [-], 765XerD-like tyrosine recombinase-2 family proteinIntegrase 
12MHH_c226402136369..2138348 [-], 1980integrating conjugative element relaxaseRelaxase, MOBH Family
13MHH_c226502138776..2139573 [-], 798putative HsdM-like type I restriction-modification system methyltransferase subunit
14MHH_c226602139646..2140392 [-], 747hypothetical protein
15MHH_c226702140532..2141506 [-], 975antirestriction protein
16MHH_c226802141751..2142158 [-], 408hypothetical protein
17MHH_c226902142274..2143116 [-], 843hypothetical protein
18MHH_c227002143194..2143589 [-], 396hypothetical protein
19MHH_c227102143688..2143933 [-], 246hypothetical protein
20MHH_c227202144005..2144358 [-], 354hypothetical protein
21MHH_c227302144732..2145175 [+], 444DUF1525 domain-containing integrating conjugative element proteinTfc24, T4SS component 
22MHH_c227402145172..2146113 [+], 942TraU-like integrating conjugative element proteinTfc23, T4SS component 
23MHH_c227502146131..2148143 [+], 2013integrating conjugative element proteinTfc22, T4SS component 
24MHH_c227602148164..2148592 [+], 429hypothetical proteinTfc21, T4SS component 
25MHH_c227702148808..2149278 [+], 471hypothetical protein
26MHH_c227802149299..2149640 [+], 342hypothetical proteinTfc20, T4SS component 
27MHH_c227902149686..2151158 [-], 1473TraG-like proteinTfc19, T4SS component 
28MHH_c228002151169..2151486 [-], 318hypothetical proteinTfc18, T4SS component 
29MHH_c228102151476..2151904 [-], 429hypothetical proteinTfc17, T4SS component 
30MHH_c228202151904..2154741 [-], 2838TraC-like conjugative transfer ATPaseTfc16, T4SS component 
31MHH_c228302154744..2155142 [-], 399conjugative transfer region lipoproteinTfc15, T4SS component 
32MHH_c228402155155..2156609 [-], 1455integrating conjugative element proteinTfc14, T4SS component 
33MHH_c228502156621..2157505 [-], 885TraK-like DUF3438 domain-containing integrating conjugative element proteinTfc13, T4SS component 
34MHH_c228602157505..2158149 [-], 645DUF2895 domain-containing integrating conjugative element proteinTfc12, T4SS component 
35MHH_c228702158153..2158518 [-], 366DUF3487 domain-containing conjugative transfer region proteinTfc11, T4SS component 
36MHH_c228802158541..2158930 [-], 390DUF2976 domain-containing integrating conjugative element proteinTfc10, T4SS component 
37MHH_c228902158945..2159178 [-], 234hypothetical protein DUF3262
38MHH_c229002159178..2159504 [-], 327integrative conjugative element proteinTfc9, T4SS component 
39MHH_c229102159691..2160074 [+], 384hypothetical protein
40MHH_c229202160106..2160801 [-], 696DUF4400 domain-containing integrating conjugative element membrane proteinTfc8, T4SS component 
41MHH_c229302160798..2161151 [-], 354hypothetical proteinTfc7, T4SS component 
42MHH_c229402161144..2163345 [-], 2202TraD-like conjugative coupling factor, SXT/TOL subfamilyTfc6, T4SS component 
43MHH_c229502163345..2163851 [-], 507DUF2859 domain-containing integrating conjugative element proteinTfc5, T4SS component 
44MHH_c229602163854..2164624 [-], 771putative lysozyme-like superfamilyTfc4, T4SS component 
45MHH_c229702164603..2165376 [-], 774integrating conjugative element proteinTfc3, T4SS component 
46MHH_c229802165376..2166005 [-], 630PilL-like conjugative transfer region proteinTfc2, T4SS component 
47MHH_c229902166164..2166424 [-], 261hypothetical protein
48MHH_c230002166537..2166899 [-], 363hypothetical protein
49MHH_c230102166979..2167386 [-], 408hypothetical protein
50MHH_c230202167412..2168092 [-], 681hypothetical protein
51MHH_c230302168198..2168629 [-], 432hypothetical protein DUF3577
52topB12169447..2171498 [-], 2052DNA topoisomerase 3 TopB
53MHH_c230502171658..2172176 [-], 519hypothetical protein
54MHH_c230602172273..2172557 [-], 285hypothetical protein
55MHH_c230702172653..2173363 [+], 711hypothetical protein
56MHH_c230802173902..2174669 [-], 768Tas-like aldo/keto reductase
57MHH_c230902174668..2174988 [+], 321hypothetical protein
58MHH_c231002175170..2175625 [+], 456putative DUF411 domain-containing metal-binding protein
59mco2175638..2177185 [+], 1548multicopper oxidase Mco
60MHH_c231202177275..2177430 [-], 156zinc-binding domain-containing alcohol dehydrogenase
61MHH_c231302177519..2177698 [+], 180hypothetical protein
62MHH_c231402177747..2178265 [+], 519putative NADH kinase
63tetR2178268..2178891 [-], 624tetracycline repressor protein TetR
64tetH2178983..2180185 [+], 1203tetracycline efflux protein, class HAR 
65MHH_c231702180281..2181591 [-], 1311ISPst9-like transposase
66MHH_c231902181591..2181893 [-], 303hypothetical protein
67MHH_c232002181934..2182122 [-], 189hypothetical protein
68ssb32182429..2182881 [-], 453single-stranded DNA-binding protein Ssb
69MHH_c232202183237..2183722 [-], 486hypothetical protein DUF3158
70MHH_c232302183731..2184489 [-], 759DUF1845 domain-containing integrating conjugative element protein
71MHH_c232402184668..2185858 [-], 1191hypothetical protein
72MHH_c232502186017..2186574 [-], 558hypothetical protein DUF2857
73MHH_c232602186567..2188213 [-], 1647ParB-like integrating conjugative element protein
74dnaB22188222..2189583 [-], 1362replicative DNA helicase DnaB
75MHH_c232802189595..2190419 [-], 825chromosome partitioning ATPase
76xseA2190914..2192374 [-], 1461exodeoxyribonuclease VII - large subunit XseA
77mltA2192811..2193917 [+], 1107membrane-bound lytic murein transglycosylase MltA
78MHH_c233302193930..2194379 [+], 450hypothetical protein
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins71Fasta
(1) Eidam C; Poehlein A; Leimbach A; Michael GB; Kadlec K; Liesegang H; Daniel R; Sweeney MT; Murray RW; Watts JL; Schwarz S (2015). Analysis and comparative genomics of ICEMh1, a novel integrative and conjugative element (ICE) of Mannheimia haemolytica. J Antimicrob Chemother. 70(1):93-7. [PudMed:25239467]
(2) Klima CL; Zaheer R; Cook SR; Booker CW; Hendrick S; Alexander TW; McAllister TA (2014). Pathogens of bovine respiratory disease in North American feedlots conferring multidrug resistance via integrative conjugative elements. J Clin Microbiol. 52(2):438-48. [PudMed:24478472]