ICEberg
I. Information of ICE
ICEberg ID861
Name Tn6194-like This ICE is derived from experimental literature
FamilyUnclassified
OrganismClostridium difficile CII7
Size (bp)28014
GC content [Genome] (%)39.06
Insertion site7 different instertion sites in Clostridium difficile ;tRNAArg(in Enterococcus faecalis)
FunctionAntibiotic resistance genes: erm(B)
Species that ICE can be transferred toClostridium difficile; Enterococcus faecalis
Nucleotide SequenceHG475346 (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..28014 
Putative oriT region -
Putative relaxase coordinates: 6900..8096;   Family:  MOBT


II. ICE interaction with IME/CIME/

The interaction information of Tn6194-like is not available.



The graph information of Tn6194-like components from HG475346
Complete gene list of Tn6194-like from HG475346
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1-302..3346 [+], 3045cell surface protein
2-3579..3905 [+], 327conjugative transposon protein
3-3921..4304 [+], 384conjugative transposon protein, Tn916 orf22-like
4-4472..4879 [+], 408transcriptional regulator
5-4895..5221 [+], 327hypothetical protein
6-5322..6716 [+], 1395conjugative transposon FtsK/SpoIIIE-like protein,Tn916 orf21-likeOrf21_Tn, T4SS component 
7-6900..8096 [+], 1197conjugative transposon replication initiation factor, Tn916 orf20-likeRelaxase, MOBT Family
8-8109..8243 [+], 135conjugative transposon protein
9-8244..8465 [+], 222conjugative transposon protein, Tn916 orf19-likeOrf19_Tn, T4SS component 
10-8669..9445 [+], 777hypothetical protein
11-9530..9826 [+], 297conjugative transposon protein
12-9961..10248 [+], 288conjugative transposon protein, ArdA-like
13-10266..10769 [+], 504antirestriction protein
14-10887..11285 [+], 399conjugative transposon membrane protein, Tn916 orf17-likeOrf17_Tn, T4SS component 
15-11263..13713 [+], 2451conjugative transposon protein, Tn916 orf16-likeOrf16_Tn, T4SS component 
16-13710..15908 [+], 2199conjugative transposon protein, Tn916 orf15-likeOrf15_Tn, T4SS component 
17-15905..16912 [+], 1008conjugative transposon protein, Tn916 orf14-likeOrf14_Tn, T4SS component 
18-16928..17839 [+], 912conjugative transposon protein, Tn916 orf13-likeOrf13_Tn, T4SS component 
19-17960..18337 [+], 378transcriptional regulator
20-18338..19201 [+], 864ATP binding cassette transporter protein
21-19198..19842 [+], 645hypothetical protein
22-19855..20046 [+], 192hypothetical protein
23-20080..20241 [+], 162hypothetical protein
24-20308..20439 [+], 132hypothetical protein
25-20547..20906 [-], 360DNA-binding helix-turn-helix protein
26-21099..21308 [+], 210transcriptionnal repressor
27-21325..21597 [+], 273antitoxin
28-21599..21757 [+], 159toxin
29-21809..22228 [+], 420hypothetical protein
30ermB22275..23012 [+], 738erythromycin ribosomal methylaseAR 
31-23587..24423 [+], 837putative ATPase
32-24871..25287 [+], 417hypothetical protein
33-25294..25542 [+], 249hypothetical protein
34xis25937..26194 [+], 258excisionase
35int26213..27658 [+], 1446integraseIntegrase 
 
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins35Fasta
(1) Wasels F; Monot M; Spigaglia P; Barbanti F; Ma L; Bouchier C; Dupuy B; Mastrantonio P (2014). Inter- and intraspecies transfer of a Clostridium difficile conjugative transposon conferring resistance to MLSB. Microb Drug Resist. 20(6):555-60. [PudMed:25055190]
(2) Wasels F; Spigaglia P; Barbanti F; Mastrantonio P (2013). Clostridium difficile erm(B)-containing elements and the burden on the in vitro fitness. J Med Microbiol. 62(Pt 9):1461-7. [PudMed:23741023]