ICEberg
I. Information of ICE
ICEberg ID827
Name ICESpy009 This ICE is derived from experimental literature
FamilyICESa2603
OrganismStreptococcus pyogenes MB56Spyo009
Size (bp)55,259
GC content [Genome] (%)39.56
Insertion site-
FunctionAntibiotic resistance genes: mef(E)
Species that ICE can be transferred toStreptococcus pyogenes; Streptococcus pneumoniae
Nucleotide SequenceKU056701. (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..55259 
Putative oriT region -
Putative relaxase coordinates: 37025..38887;   Family:  MOBP


II. ICE interaction with IME/CIME/

The interaction information of ICESpy009 is not available.



The graph information of ICESpy009 components from KU056701
Complete gene list of ICESpy009 from KU056701
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1-264..437 [+], 174hypothetical protein
2rep434..1258 [+], 825Replication initiator protein
3-1416..2771 [+], 1356DNA-cytosine methyltransferase
4-2755..3183 [+], 429hypothetycal protein
5-3193..3576 [+], 384ArsC family protein
6-3586..3819 [+], 234hypothetical protein
7-3822..4403 [+], 582hypothetical protein
8-4480..4968 [+], 489hypothetical protein
9virD44968..6788 [+], 1821putative Type IV secretion protein VirD4T4CP 
10-6806..7048 [+], 243membrane protein
11virB67065..7919 [+], 855putative Type IV secretion protein VirB6PrgH, T4SS component 
12-7981..8334 [+], 354hypothetical protein
13virB48291..10642 [+], 2352putative Type IV secretion protein VirB4PrgJ, T4SS component 
14virB1-like10639..13443 [+], 2805putative Type IV secretion protein VirB1-like proteinPrgK, T4SS component 
15-13688..14281 [+], 594hypothetical protein
16-14525..19429 [+], 4905glucan binding proteinPrgB, T4SS component 
17-19430..19621 [+], 192calcium binding protein
18-19605..20156 [+], 552hypothetical protein
19-20206..27027 [+], 6822SNF2 family protein
20-27097..27396 [+], 300hypothetical protein
21-27411..27701 [+], 291membrane protein
22-27812..28450 [+], 639hypothetical proteinPrgL, T4SS component 
23-28490..29578 [+], 1089DNA primase
24-29632..29847 [+], 216hypothetical protein
25-29863..30258 [+], 396chemotaxis protein
26-30302..30589 [+], 288ATPase
27-30616..30915 [-], 300hypothetical protein
28-30902..31270 [-], 369hypothetical protein
29-31267..32685 [-], 1419DNA-directed DNA polymerase
30-32687..32821 [-], 135hypothetical protein
31-32824..33516 [-], 693DNA binding protein
32-33730..34800 [+], 1071DNA recombination protein
33-34833..35687 [-], 855transposaseIntegrase 
34-36172..36285 [+], 114hypothetical protein
35-36304..36663 [+], 360hypothetical protein
36-36673..37038 [+], 366hypothetical protein
37-37025..38887 [+], 1863relaxaseRelaxase, MOBP Family
38-38923..39084 [+], 162hypothetical protein
39-39653..39838 [+], 186hypothetical protein
40mef(E)40224..41441 [+], 1218Macrolide efflux protein EAR 
41mel41561..43024 [+], 1464Mel protein
42-43142..43441 [-], 300hypothetical protein
43-43428..43796 [-], 369hypothetical protein
44-43809..44144 [-], 336hypothetical protein
45-44141..44386 [-], 246ImpB/MucB/SamB family protein
46-44383..44571 [-], 189hypothetical protein
47-45351..45821 [+], 471transcriptional regulator
48-45805..46494 [+], 690putative aminoglycoside phosphotransferase
49-46498..46830 [+], 333putative aminoglycoside phosphotransferase
50-46827..48119 [+], 1293putative ABC transporter
51-48258..48572 [+], 315putative ABC transporter
52-48553..50292 [+], 1740putative ABC transporter
53-50404..50625 [-], 222putative transposase
54-50689..51183 [+], 495putative peptidase
55-51439..51606 [-], 168hypothetical protein
56-52222..52437 [-], 216hypothetical protein
57-52334..52843 [+], 510transposase
58-52840..53154 [+], 315transposase
59-53265..53483 [+], 219Cro/CI family transcriptional regulator
60-53687..54001 [+], 315hypothetical protein
61int54004..55209 [+], 1206integrase
 
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins61Fasta
(1) Del Grosso M; Camilli R; Rizzi E; Pietrelli A; De Bellis G; Pantosti A (2016). ICESpy009, a Conjugative Genetic Element Carrying mef(E) in Streptococcus pyogenes. Antimicrob Agents Chemother. 60(7):3906-12. [PudMed:27067338] in_silico
 
in_silico in silico analysis literature