ICEberg
I. Information of ICE
ICEberg ID816
Name ICESpn529IQ This is a predicted ICE derived from literature
FamilyTn5253
OrganismStreptococcus pneumoniae Spn529
Size (bp)59,466
GC content [Genome] (%)35.81
Insertion site3' end of gene rplL
FunctionAntibiotic resistance genes: mef(I), catQ
Species that ICE can be transferred to-
Nucleotide SequenceHG965092 (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..59466 
Putative oriT region -
Putative relaxase coordinates: 2176..4005;   Family:  MOBP


II. ICE interaction with IME/CIME/

The interaction information of ICESpn529IQ is not available.



The graph information of ICESpn529IQ components from HG965092
Complete gene list of ICESpn529IQ from HG965092
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1int147..1334 [-], 1188integrase
2-1306..1614 [-], 309hypothetical protein
3-1845..2072 [-], 228putative transcriptional regulator
4-2176..4005 [-], 1830relaxaseRelaxase, MOBP Family
5-3992..4357 [-], 366mobilisation protein
6-4362..4718 [-], 357mobilisation protein
7-5632..7845 [-], 2214hypothetical protein
8pezT8158..8919 [-], 762zeta toxin PezT
9pezA8919..9395 [-], 477antitoxin PezA
10-9463..9735 [-], 273ATPases with chaperone activity
11-9773..10156 [-], 384methyl-accepting chemotaxis protein
12-10153..10386 [-], 234hypothetical protein
13-10437..11522 [-], 1086hypothetical protein
14-11532..12173 [-], 642hypothetical protein
15-12333..12530 [+], 198hypothetical protein
16-12544..12840 [-], 297hypothetical protein
17-12854..13138 [-], 285hypothetical protein
18-13213..19446 [-], 6234SNF2 family protein
19-19479..19889 [-], 411hypothetical protein
20-20088..20729 [-], 642ABC_ATPases superfamily
21-20731..22752 [-], 2022hypothetical protein
22tnp123013..24329 [+], 1317transposase
23catQ25029..25688 [-], 660chloramphenicol acetyltransferaseAR 
24nf1126010..26510 [-], 501hypothetical protein
25nf1026497..27078 [-], 582putative flavin oxidoreductase
26nf927196..27663 [-], 468hypothetical protein
27msrD27795..29258 [-], 1464ABC-transporter protein
28mefI29373..30590 [-], 1218macrolide-efflux protein IAR 
29rec231022..32593 [-], 1572putative recombinase
30nf532597..33046 [-], 450hypothetical protein
31-33010..33507 [-], 498hypothetical protein
32-33778..34242 [-], 465putative recombinase
33-34625..34966 [-], 342hypothetical protein
34nf335347..35952 [-], 606hypothetical protein
35hol35918..36478 [-], 561phage holin protein
36tnp136527..37843 [+], 1317putative transposase
37tetM37965..39899 [+], 1935tetM proteinAR 
38-39991..40185 [+], 195hypothetical protein
39-40245..40598 [-], 354hypothetical protein
40-40826..41146 [+], 321hypothetical protein
41-41103..41525 [+], 423putative sigma factor
42-41522..41752 [+], 231hypothetical protein
43-41978..42229 [-], 252hypothetical protein
44xis-Tn91642213..42416 [+], 204excisionase
45int-Tn91642498..43715 [+], 1218integraseIntegrase 
46-44103..44771 [-], 669hypothetical proteinPrgK, T4SS component 
47-45381..46928 [-], 1548hypothetical protein
48-46940..47911 [-], 972VirB4PrgJ, T4SS component 
49-48104..49297 [-], 1194hypothetical proteinPrgJ, T4SS component 
50-49248..49607 [-], 360PrgI superfamily
51-49661..50515 [-], 855hypothetical proteinPrgH, T4SS component 
52-50532..50774 [-], 243hypothetical protein
53-50795..51283 [-], 489hypothetical proteinT4CP 
54-51393..52676 [-], 1284VirD4
55-52673..53080 [-], 408hypothetical protein
56-53151..53450 [-], 300hypothetical protein
57-53732..54115 [-], 384hypothetical protein
58-54204..54563 [-], 360hypothetical protein
59-54563..55207 [-], 645hypothetical protein
60-55282..55869 [-], 588caax protease
61-55872..56105 [-], 234hypothetical protein
62-56118..56498 [-], 381Thioredoxin-like superfamily
63-56491..56940 [-], 450hypothetical protein
64-56924..58282 [-], 1359cytosine methyltransferase
65repA58381..59160 [-], 780replication protein A
66-59157..59330 [-], 174hypothetical protein
 
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins66Fasta
(1) Morici E; Simoni S; Brenciani A; Giovanetti E; Varaldo PE; Mingoia M (2017). A new mosaic integrative and conjugative element from Streptococcus agalactiae carrying resistance genes for chloramphenicol (catQ) and macrolides [mef(I) and erm(TR)]. J Antimicrob Chemother. 72(1):64-67. [PudMed:27621174] experimental
(2) Mingoia M; Morici E; Brenciani A; Giovanetti E; Varaldo PE (2014). Genetic basis of the association of resistance genes mef(I) (macrolides) and catQ (chloramphenicol) in streptococci. Front Microbiol. 0.727083333. [PudMed:25610433]
(3) Mingoia M; Morici E; Morroni G; Giovanetti E; Del Grosso M; Pantosti A; Varaldo PE (2014). Tn5253 family integrative and conjugative elements carrying mef(I) and catQ determinants in Streptococcus pneumoniae and Streptococcus pyogenes. Antimicrob Agents Chemother. 58(10):5886-93. [PudMed:25070090] experimental in_silico
 
experimental experimental literature
in_silico in silico analysis literature