ICEberg
I. Information of ICE
ICEberg ID812
Name ICESp1116 This ICE is derived from experimental literature
FamilyUnclassified
OrganismStreptococcus pyogenes A-3
Size (bp)49160
GC content [Genome] (%)35.24
Insertion siterpmH
FunctionAntibiotic resistance genes: erm(B), a truncated tet(M) gene
Species that ICE can be transferred toStreptococcus pyogenes
Nucleotide SequenceHE802677 (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..49160 
Putative oriT region -
Putative relaxase coordinates: 30613..32241;   Family:  MOBP


II. ICE interaction with IME/CIME/

The interaction information of ICESp1116 is not available.



The graph information of ICESp1116 components from HE802677
Complete gene list of ICESp1116 from HE802677
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1-147..269 [+], 123hypothetical protein
2-372..533 [+], 162hypothetical protein
3-536..1450 [+], 915replication initiator protein A
4-1437..1601 [+], 165hypothetical protein
5-1791..2165 [+], 375hypothetical protein
6-2176..2985 [+], 810hypothetical protein
7-3009..6404 [+], 3396hypothetical proteinPrgB, T4SS component 
8-6545..9907 [+], 3363peptidase family C39 proteinPrgA, T4SS component 
9-10099..10326 [+], 228hypothetical proteinPrgF, T4SS component 
10-10374..10949 [+], 576hypothetical protein
11-11001..11753 [+], 753hypothetical proteinPrgH, T4SS component 
12-11763..12224 [+], 462transcriptional regulator
13-12332..12688 [+], 357hypothetical protein
14-12633..15023 [+], 2391type IV secretory pathway, VirB4 componentsPrgJ, T4SS component 
15-15060..17723 [+], 2664CHAP domain proteinPrgK, T4SS component 
16-17739..18344 [+], 606hypothetical proteinPrgL, T4SS component 
17-18357..18743 [+], 387single-strand DNA-binding protein
18-18765..19040 [+], 276hypothetical protein
19-19040..19834 [+], 795hypothetical protein
20-19846..20313 [+], 468hypothetical protein
21-20335..22404 [+], 2070TraG family proteinT4CP 
22-22440..22583 [+], 144hypothetical protein
23-22632..23126 [+], 495transcriptional regulator
24-23139..24920 [+], 1782DNA topoisomerase
25-24936..29600 [+], 4665topoisomerase-primase, domain protein
26-29658..29945 [+], 288hypothetical protein
27-30267..30641 [+], 375hypothetical protein
28-30613..32241 [+], 1629relaxaseRelaxase, MOBP Family
29-32277..33074 [+], 798hypothetical protein
30-33183..33353 [+], 171hypothetical protein
31-33367..33984 [+], 618hypothetical protein
32-33984..36083 [+], 2100DNA topoisomerase
33-36231..37127 [+], 897ParA protein
34-37219..37434 [+], 216omega transcriptional repressor
35-37451..37723 [+], 273bacterial epsilon antitoxin protein
36-37725..38588 [+], 864bacterial zeta toxin protein
37erm(B)38849..39586 [-], 738rRNA methylaseAR 
38-39711..39794 [-], 84rRNA methylase leader peptide
39tnp39922..40608 [-], 687transposase
40tet(M)40677..42227 [+], 1551tetracycline-resistance proteinAR 
41-42380..42517 [+], 138hypothetical protein
42-42570..42929 [-], 360transcriptional regulator
43-43431..43856 [+], 426RNA polymerase sigma-70 region 4, family protein
44-43853..44083 [+], 231hypothetical protein
45tndX44660..46291 [+], 1632site-specific recombinase
46-46768..48120 [+], 1353hypothetical proteinIntegrase 
47rpmH48551..48685 [+], 135ribosomal protein L34
 
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins47Fasta
(1) Santoro F; Vianna ME; Roberts AP (2014). Variation on a theme; an overview of the Tn916/Tn1545 family of mobile genetic elements in the oral and nasopharyngeal streptococci. Front Microbiol. 0.579861111. [PudMed:25368607]
(2) Brenciani A; Tiberi E; Morroni G; Mingoia M; Varaldo PE; Giovanetti E (2014). ICESp1116, the genetic element responsible for erm(B)-mediated, inducible erythromycin resistance in Streptococcus pyogenes, belongs to the TnGBS family of integrative and conjugative elements. Antimicrob Agents Chemother. 58(4):2479-81. [PudMed:24449773]
(3) Brenciani A; Bacciaglia A; Vecchi M; Vitali LA; Varaldo PE; Giovanetti E (2007). Genetic elements carrying erm(B) in Streptococcus pyogenes and association with tet(M) tetracycline resistance gene. Antimicrob Agents Chemother. 51(4):1209-16. [PudMed:17261630] experimental
 
experimental experimental literature