ICEberg
I. Information of ICE
ICEberg ID772
Name ICE-emm12 This is a predicted ICE derived from literature
FamilyUnclassified
OrganismStreptococcus pyogenes emm12 HKU16
Size (bp)64966
GC content [Genome] (%)36.51
Insertion site-
FunctionAntibiotic resistance genes: tet(O), tet(M), erm(B), ermTR
Species that ICE can be transferred to-
Nucleotide SequenceAFRY01000001 (complete ICE sequence in this genome)
Replicon-
Genome coordinates818690..883657 
Putative oriT region -
Putative relaxase coordinates: 850428..851789; Locus tag: SPYOHK_04140;  Family:  MOBT


II. ICE interaction with IME/CIME/

The interaction information of ICE-emm12 is not available.



The graph information of ICE-emm12 components from AFRY01000001
Complete gene list of ICE-emm12 from AFRY01000001
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1SPYOHK_03980813791..813949 [+], 159hypothetical protein
2SPYOHK_03985813956..814237 [+], 282membrane protein
3SPYOHK_03990814328..815098 [+], 771replication initiation protein
4SPYOHK_03995815095..815946 [+], 852hypothetical protein
5SPYOHK_04000815943..816428 [+], 486hypothetical protein
6SPYOHK_04005816425..818209 [+], 1785putative conjugal transfer protein
7SPYOHK_04010818375..818686 [+], 312single-stranded DNA binding protein
8SPYOHK_04015818690..818905 [+], 216conjugative transposon membrane protein
9SPYOHK_04020818925..819788 [+], 864conjugative transposon membrane protein
10SPYOHK_04025819806..820069 [+], 264conjugative transposon membrane exported protein
11SPYOHK_04030820073..820462 [+], 390conjugative transposon membrane protein
12SPYOHK_04035820374..821459 [+], 1086conjugal transfer protein
13SPYOHK_04040822161..824035 [+], 1875group II intron reverse transcriptase/maturase
14SPYOHK_04045824054..825559 [+], 1506conjugal transfer proteinPrgJ, T4SS component 
15SPYOHK_04050825564..827696 [+], 2133membrane protein
16SPYOHK_04055827709..827945 [+], 237hypothetical protein
17SPYOHK_04060827923..830109 [+], 2187cell surface protein
18SPYOHK_04065830207..831913 [+], 1707conjugative transposon DNA topoisomerase
19SPYOHK_04070831997..832176 [+], 180regulatory protein
20SPYOHK_04075832166..833623 [+], 1458DNA-binding protein
21SPYOHK_04080833710..842466 [+], 8757helicase
22SPYOHK_04085842509..843168 [+], 660conjugative transposon protein
23SPYOHK_04090843242..844573 [-], 1332conjugative transposon mobilization protein
24SPYOHK_04095844579..844929 [-], 351hypothetical protein
25SPYOHK_04100845269..845859 [+], 591TetR family transcriptional regulator
26SPYOHK_04105845871..846458 [+], 588hypothetical protein
27SPYOHK_04110846479..847180 [+], 702hypothetical protein
28SPYOHK_04115847173..847739 [+], 567ABC cobalt transporter ATPase component
29SPYOHK_04120847921..848040 [+], 120hypothetical protein
30SPYOHK_04125848063..848377 [+], 315Tn916 ORF23 protein
31SPYOHK_04130848393..848779 [+], 387Tn916 ORF22 protein
32SPYOHK_04135848808..850193 [+], 1386Tn916 ORF21 FtsK/SpoIIIE family proteinOrf21_Tn, T4SS component 
33SPYOHK_04140850428..851789 [+], 1362Cro/CI family transcriptional regulatorRelaxase, MOBT Family
34SPYOHK_04145851838..851921 [+], 84leader peptide
35SPYOHK_04150852046..852783 [+], 738erythromycin resistance proteinAR 
36SPYOHK_04155852788..852919 [+], 132hypothetical protein
37SPYOHK_04160853040..854287 [-], 1248transposase
38SPYOHK_04165854467..854688 [+], 222Tn916 ORF19 proteinOrf19_Tn, T4SS component 
39SPYOHK_04170854805..855302 [+], 498Tn916 ORF18 antirestriction (ArdA) protein
40SPYOHK_04175855277..855783 [+], 507Tn916 ORF17 signal peptide containing proteinOrf17_Tn, T4SS component 
41SPYOHK_04180855767..858214 [+], 2448hypothetical proteinOrf16_Tn, T4SS component 
42SPYOHK_04185858217..860394 [+], 2178Tn916 ORF15 signal peptide containing proteinOrf15_Tn, T4SS component 
43SPYOHK_04190860391..861392 [+], 1002Tn916 ORF14 extracellular hydrolase/peptidaseOrf14_Tn, T4SS component 
44SPYOHK_04195861389..862321 [+], 933Tn916 ORF13 proteinOrf13_Tn, T4SS component 
45SPYOHK_04200862566..862682 [+], 117tetracycline resistance determinant leader peptide
46SPYOHK_04205862698..864617 [+], 1920Tn916 ORF11 tetracycline resistance proteinAR 
47SPYOHK_04210864736..864903 [+], 168hypothetical protein
48SPYOHK_04215864963..865316 [-], 354Tn916 ORF9 transcriptional regulator, putative
49SPYOHK_04220865821..866243 [+], 423Tn916 ORF7 DNA-binding protein
50SPYOHK_04225866240..866470 [+], 231hypothetical protein
51SPYOHK_04230866931..867134 [+], 204Tn916 ORF1 excisionase
52SPYOHK_04235867216..868433 [+], 1218Tn916 ORF2 integraseIntegrase 
53SPYOHK_04240868621..869616 [-], 996Integrase catalytic region
54SPYOHK_04245869763..870557 [+], 795ABC cobalt transporter ATPase component
55SPYOHK_04250870591..872336 [+], 1746multidrug ABC transporter
56SPYOHK_04255872330..874063 [+], 1734multidrug ABC transporter
57SPYOHK_04260874099..875457 [+], 1359Na+driven multidrug efflux pump
58SPYOHK_04265875801..876211 [+], 411hypothetical protein
59SPYOHK_04270876668..876811 [+], 144hypothetical protein
60SPYOHK_04275876876..878675 [+], 1800recombinase
61SPYOHK_04280878709..878801 [+], 93hypothetical protein
62SPYOHK_04285878992..879192 [+], 201phosphorylase Pnp/Udp family protein
63SPYOHK_04290879303..879503 [+], 201phosphorylase
64SPYOHK_04295879588..879989 [+], 402thioesterase superfamily protein
65SPYOHK_04300880094..881578 [-], 1485hypothetical protein
66SPYOHK_04305881952..883172 [+], 1221major facilitator transporter
67SPYOHK_04310883292..883657 [-], 366putative thioesterase
68SPYOHK_04315883768..884502 [+], 735ribosomal small subunit pseudouridine synthase B
69SPYOHK_04320884551..885357 [-], 807transposase
70SPYOHK_04325885378..885893 [-], 516transposase
71SPYOHK_04330886135..886263 [+], 129hypothetical protein
72obgE886326..887633 [+], 1308GTPase CgtA
73SPYOHK_04340887702..887860 [+], 159hypothetical protein
74SPYOHK_04345887865..888572 [-], 708nicotinamide mononucleotide transporter
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer
virulence  Gene may be involved in adaptative function

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins60Fasta
(1) Andrey DO; Posfay-Barbe KM (2016). Re-emergence of scarlet fever: old players return?. Expert Rev Anti Infect Ther. 14(8):687-9. [PudMed:27249582]
(2) Davies MR; Holden MT; Coupland P; Chen JH; Venturini C; Barnett TC; Zakour NL; Tse H; Dougan G; Yuen KY; Walker MJ (2015). Emergence of scarlet fever Streptococcus pyogenes emm12 clones in Hong Kong is associated with toxin acquisition and multidrug resistance. Nat Genet. 47(1):84-7. [PudMed:25401300] experimental in_silico
(3) Tse H; Bao JY; Davies MR; Maamary P; Tsoi HW; Tong AH; Ho TC; Lin CH; Gillen CM; Barnett TC; Chen JH; Lee M; Yam WC; Wong CK; Ong CL; Chan YW; Wu CW; Ng T; Lim WW; Tsang TH; Tse CW; Dougan G; Walker MJ; Lok S; Yuen KY (2012). Molecular characterization of the 2011 Hong Kong scarlet fever outbreak. J Infect Dis. 206(3):341-51. [PudMed:22615319]
 
experimental experimental literature
in_silico in silico analysis literature