ICEberg
I. Information of ICE
ICEberg ID59
Name ICEBs1 This ICE is derived from experimental literature
FamilyICEBs1
OrganismBacillus subtilis subsp. subtilis str. 168
Size (bp)20510
GC content [Genome] (%)35.7[43.51]
Insertion sitetRNA-Leu(BSU_tRNA_51)
Function-
Species that ICE can be transferred toBacillus and Listeria species
Nucleotide SequenceAL009126 (complete ICE sequence in this genome)
Replicon chromosome (4215606 bp, BioProject:76) [NC_000964]
Genome coordinates529423..549932 
Putative oriT region -
Putative relaxase coordinates: 534773..535831; Locus tag: BSU04870;  Family:  MOBT


II. ICE interaction with IME/CIME/

The interaction information of ICEBs1 is not available.



The graph information of ICEBs1 components from AL009126
Complete gene list of ICEBs1 from AL009126
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1ydcF524492..524785 [+], 294hypothetical protein
2ydcG524782..525222 [+], 441conserved hypothetical protein
3ydcH525206..525649 [+], 444putative transcriptional regulator
4ydcI525743..527902 [+], 2160putative RNA helicase
5ydcK528129..528581 [+], 453conserved hypothetical protein
6ydcL (int)529505..530611 [-], 1107putative phage integraseIntegrase 
7immA530624..531133 [-], 510immunity anti-repressor conserved in prophages
8immR531130..531513 [-], 384phage element (ICEBs1)transcriptional regulator (Xre family)
9sacV (xis)531787..531981 [+], 195transcriptional regulator
10ydzL531978..532238 [+], 261conserved hypothetical protein
11ydcO532292..532552 [+], 261hypothetical protein; mobile element region
12ydcP (helP)532922..533302 [+], 381conserved hypothetical protein; mobile element regionHelP; ICEBs1-encoded helicase processivity factor; [PMID: 23326247
13ydcQ (conQ)533338..534780 [+], 1443putative DNA wielding protein; mobile element regionYdcQ, T4SS component 
14ydcR (nicK)534773..535831 [+], 1059putative replication protein; mobile element regionRelaxase, MOBT Family
15ydcS536096..536365 [+], 270conserved hypothetical protein; mobile element region
16ydcT536404..536670 [+], 267conserved hypothetical protein; mobile element region
17yddA536687..536995 [+], 309conserved hypothetical protein; mobile element region
18yddB (conB)536985..538049 [+], 1065conserved hypothetical protein; mobile element regionYddB, T4SS component 
19yddC (conC)538061..538309 [+], 249conserved hypothetical proteinYddC, T4SS component 
20yddD (conD)538322..538846 [+], 525hypothetical protein; mobile element regionYddD, T4SS component 
21yddE (conE)538836..541229 [+], 2394conserved hypothetical proteinYddE, T4SS component 
22yddF541248..541574 [+], 327conserved hypothetical protein; mobile element region
23yddG (conG)541578..544025 [+], 2448conserved hypothetical protein; mobile element regionYddG, T4SS component 
24yddH (cwlT)544022..545011 [+], 990putative cell wall hydrolase; mobile element regionYddH, T4SS component 
25yddI545026..545532 [+], 507conserved hypothetical protein; mobile element region
26yddJ545595..545975 [+], 381putative lipoprotein
27yddK546166..546966 [-], 801conserved hypothetical protein; mobile element region
28rapI547306..548481 [+], 1176response regulator aspartate phosphatase; mobile element region
29phrI548438..548557 [+], 120secreted regulator of the activity of phosphatase RapI
30yddM548710..549651 [+], 942putative helicase; mobile element region
31yddN550240..551259 [-], 1020putative alkanal monooxygenase
32lrpA551519..551929 [+], 411transcriptional regulator (Lrp/AsnC family)
33lrpB552052..552501 [-], 450transcriptional regulator (Lrp/AsnC family)
34yddQ552616..553158 [+], 543putative hydrolase
35yddR553711..554475 [+], 765putative metal-dependent hydrolase
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins25Fasta
(1) Lee CA; Thomas J; Grossman AD (2012). The Bacillus subtilis conjugative transposon ICEBs1 mobilizes plasmids lacking dedicated mobilization functions. J Bacteriol. 194(12):3165-72. [PudMed:22505685] experimental
(2) Babic A; Berkmen MB; Lee CA; Grossman AD (2011). Efficient gene transfer in bacterial cell chains. MBio. 2(2). [PudMed:21406598] experimental
(3) Smits WK; Grossman AD (2010). The transcriptional regulator Rok binds A+T-rich DNA and is involved in repression of a mobile genetic element in Bacillus subtilis. PLoS Genet. 6(11):e1001207. [PudMed:21085634] experimental
(4) Bose B; Grossman AD (2011). Regulation of horizontal gene transfer in Bacillus subtilis by activation of a conserved site-specific protease. J Bacteriol. 193(1):22-9. [PudMed:21036995] experimental
(5) Lee CA; Babic A; Grossman AD (2010). Autonomous plasmid-like replication of a conjugative transposon. Mol Microbiol. 75(2):268-79. [PudMed:19943900] experimental
(6) Berkmen MB; Lee CA; Loveday EK; Grossman AD (2010). Polar positioning of a conjugation protein from the integrative and conjugative element ICEBs1 of Bacillus subtilis. J Bacteriol. 192(1):38-45. [PudMed:19734305] experimental
(7) Bose B; Auchtung JM; Lee CA; Grossman AD (2008). A conserved anti-repressor controls horizontal gene transfer by proteolysis. Mol Microbiol. 70(3):570-82. [PudMed:18761623] experimental
(8) Lee CA; Auchtung JM; Monson RE; Grossman AD (2007). Identification and characterization of int (integrase), xis (excisionase) and chromosomal attachment sites of the integrative and conjugative element ICEBs1 of Bacillus subtilis. Mol Microbiol. 66(6):1356-69. [PudMed:18005101] experimental
(9) Lee CA; Grossman AD (2007). Identification of the origin of transfer (oriT) and DNA relaxase required for conjugation of the integrative and conjugative element ICEBs1 of Bacillus subtilis. J Bacteriol. 189(20):7254-61. [PudMed:17693500] experimental
(10) Goranov AI; Kuester-Schoeck E; Wang JD; Grossman AD (2006). Characterization of the global transcriptional responses to different types of DNA damage and disruption of replication in Bacillus subtilis. J Bacteriol. 188(15):5595-605. [PudMed:16855250] experimental
(11) Auchtung JM; Lee CA; Monson RE; Lehman AP; Grossman AD (2005). Regulation of a Bacillus subtilis mobile genetic element by intercellular signaling and the global DNA damage response. Proc Natl Acad Sci U S A. 102(35):12554-9. [PudMed:16105942] experimental
 
experimental experimental literature