ICEberg
I. Information of ICE
ICEberg ID542
Name pCM32 {AICE} This ICE is derived from experimental literature
FamilyUnclassified
OrganismSaccharopolyspora endophytica YIM
Size (bp)14,611
GC content [Genome] (%)67.2
Insertion sitetRNA(Ser)
Functionimprove production of spinosyn in Saccharopolyspora spinosa
Species that ICE can be transferred toSaccharopolyspora spinosa
Nucleotide SequenceKM260754 (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..14611 
Putative oriT region -
Putative relaxase -


II. ICE interaction with IME/CIME/

The interaction information of pCM32 {AICE} is not available.



The graph information of pCM32 {AICE} components from KM260754
Complete gene list of pCM32 {AICE} from KM260754
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1pCM32.1c9..1238 [-], 1230hypothetical protein
2pCM32.21271..1633 [+], 363Rep3
3pCM23.3c1639..2004 [-], 366GntR family transcriptional regulator
4pCM32.42102..2410 [+], 309transcriptional regulator
5pCM32.52413..2718 [+], 306WhiB-family transcriptional regulator
6pCM32.6c2703..2939 [-], 237hypothetical protein
7pCM32.73083..3490 [+], 408hypothetical protein
8pCM32.83497..4447 [+], 951TraB
9pCM32.94466..4927 [+], 462hypothetical protein
10pCM32.10c4914..5141 [-], 228hypothetical protein
11pCM32.115207..5485 [+], 279hypothetical protein
12pCM32.125482..6780 [+], 1299hypothetical protein
13pCM32.136895..9096 [+], 2202cell division protein FtsK/SpoIIIE
14pCM32.149295..10308 [+], 1014Rep1
15pCM32.1510305..11903 [+], 1599Rep2
16pCM32.1612287..12493 [+], 207Excisionase/Xis
17pCM32.1712549..14036 [+], 1488IntegraseIntegrase 
 
integrase Gene may contribute to site-specific recombination

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins17Fasta
(1) Chen J; Xia H; Dang F; Xu Q; Li W; Qin Z (2015). Characterization of the chromosomal integration of Saccharopolyspora plasmid pCM32 and its application to improve production of spinosyn in Saccharopolyspora spinosa. Appl Microbiol Biotechnol. 99(23):10141-9. [PudMed:26260388] experimental in_silico
 
experimental experimental literature
in_silico in silico analysis literature