ICEberg
I. Information of ICE
ICEberg ID46
Name Tn4371 This ICE is derived from experimental literature
FamilyTn4371
OrganismRalstonia oxalatica A5
Size (bp)54657
GC content [Genome] (%)63.49
Insertion siteTTTTTCAT
FunctionDegrade biphenyl and 4-chlorobiphenyl
Species that ICE can be transferred toCupriavidus metallidurans
Nucleotide SequenceAJ536756 (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..54657 
Putative oriT region -
Putative relaxase coordinates: 26607..28160;   Family:  MOBL


II. ICE interaction with IME/CIME/

The interaction information of Tn4371 is not available.



The graph information of Tn4371 components from AJ536756
Complete gene list of Tn4371 from AJ536756
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1int181..1389 [+], 1209putative site specific recombinaseIntegrase 
2-1469..1765 [+], 297hypothetical protein
3-1762..2169 [+], 408hypothetical protein
4-2210..3385 [-], 1176hypothetical protein
5-3388..4533 [+], 1146putative transposase
6-4813..5835 [-], 1023hypothetical protein
7-5989..7980 [-], 1992hypothetical protein
8-8072..9871 [-], 1800hypothetical protein
9-9874..12012 [-], 2139hypothetical protein
10-12442..12792 [+], 351hypothetical protein
11-13054..13410 [-], 357hypothetical protein
12-13502..13882 [-], 381hypothetical protein
13-14290..15117 [+], 828hypothetical protein
14-15194..17266 [+], 2073hypothetical protein
15-17330..17539 [+], 210hypothetical protein
16-18015..18785 [-], 771hypothetical protein
17-18782..19276 [-], 495putative transcription regulator
18-19931..20329 [+], 399hypothetical proteinTraF, T4SS component 
19-20697..20990 [-], 294putative transcription regulator
20-21160..21561 [+], 402hypothetical protein
21-21903..22664 [+], 762hypothetical protein
22-22748..23038 [+], 291hypothetical protein
23repA23065..23922 [+], 858putative replication initiation protein
24parA24188..24826 [+], 639putative plasmid partition protein
25parB24823..25086 [+], 264hypothetical protein
26-25083..25616 [+], 534hypothetical protein
27traF25613..26212 [+], 600putative plasmid conjugal transfer transmembrane proteinTraF, T4SS component 
28-26607..28160 [+], 1554hypothetical proteinRelaxase, MOBL Family
29bphS28524..29468 [-], 945transcription regulator
30bphE29962..30768 [+], 8072-oxopent-4-enoate hydratase
31bphG30771..31712 [+], 942acetaldehyde dehydrogenase oxidoreductase
32bphF31724..32779 [+], 1056putative 4-hydroxy-2-oxovalerate aldolase transmembrane protein
33bphA133268..34644 [+], 1377biphenyl 2,3-dioxygenase oxidoreductase
34bphA234676..35257 [+], 582biphenyl 2,3-dioxygenase
35bphA335275..35604 [+], 3303-phenylpropionate dioxygenase ferredoxin oxidoreductase
36bphB35623..36453 [+], 8312,3-dihydroxy-2,3-dihydro-phenylpropionate dehydrogenase
37bphC36467..37348 [+], 8822,3-dihydroxybiphenyl dioxygenase
38bphD37380..38240 [+], 8612-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase
39-38365..39021 [+], 657hypothetical protein
40bphA439116..40411 [+], 12963-phenylpropionate dioxygenase ferredoxin oxidoreductase
41traR40563..41606 [+], 1044transcription regulator
42traG42118..44121 [+], 2004putative conjugal transfer proteinVirD4, T4SS component 
43-44118..44582 [+], 465hypothetical protein
44trbB44579..45616 [+], 1038putative mating pair formation proteinTrbB, T4SS component 
45trbC45622..46005 [+], 384putative mating pair formation transmembrane proteinTrbC, T4SS component 
46trbE46287..48740 [+], 2454putative mating pair formation proteinTrbE, T4SS component 
47trbJ48737..49492 [+], 756putative mating pair formation proteinTrbJ, T4SS component 
48-49504..49803 [+], 300hypothetical proteinTrbJ, T4SS component 
49trbL49800..51149 [+], 1350putative mating pair formation transmembrane proteinTrbL, T4SS component 
50trbF51168..51872 [+], 705putative mating pair formation proteinTrbF, T4SS component 
51trbG51869..52849 [+], 981putative mating pair formation proteinTrbG, T4SS component 
52trbI52852..54132 [+], 1281putative mating pair formation transmembrane proteinTrbI, T4SS component 
53-54129..54374 [+], 246hypothetical protein
 
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins53Fasta
(1) Toussaint A; Merlin C; Monchy S; Benotmane MA; Leplae R; Mergeay M; Springael D (2003). The biphenyl- and 4-chlorobiphenyl-catabolic transposon Tn4371, a member of a new family of genomic islands related to IncP and Ti plasmids. Appl Environ Microbiol. 69(8):4837-45. [PudMed:12902278] experimental
(2) Merlin C; Springael D; Toussaint A (1999). Tn4371: A modular structure encoding a phage-like integrase, a Pseudomonas-like catabolic pathway, and RP4/Ti-like transfer functions. Plasmid. 41(1):40-54. [PudMed:9887305] experimental
(3) Springael D; Kreps S; Mergeay M (1993). Identification of a catabolic transposon, Tn4371, carrying biphenyl and 4-chlorobiphenyl degradation genes in Alcaligenes eutrophus A5. J Bacteriol. 175(6):1674-81. [PudMed:8383664] experimental
 
experimental experimental literature