ICEberg
I. Information of ICE
ICEberg ID445
Name ICESsu32457 This ICE is derived from experimental literature
FamilyICESa2603
OrganismStreptococcus suis 32457
Size (bp)54879
GC content [Genome] (%)38.89
Insertion siteTTATTTAAGAGTAAC
FunctionCarried Tetracycline resistance determinants tet(O/W/32/O), macrolide resistance determinant erm (B) and an aminoglycoside resistance four-gene cluster including aadE (streptomycin) and aphA (kanamycin)
Species that ICE can be transferred toStreptococcus suis v36RF; Streptococcus pyogenes 12RF; Streptococcus pneumoniae R6RF; Streptococcus agalactiae 1357RF
Nucleotide SequenceFR823304 (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..54879 
Putative oriT region -
Putative relaxase coordinates: 2194..4059;   Family:  MOBP


II. ICE interaction with IME/CIME/

The interaction information of ICESsu32457 is not available.



The graph information of ICESsu32457 components from FR823304
Complete gene list of ICESsu32457 from FR823304
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1-61..1260 [-], 1200tyrosine family site-specific integraseIntegrase 
2-1283..1510 [-], 228hypothetical protein
3-1788..2012 [-], 225transcriptional regulator, Cro/CI family
4-2194..4059 [-], 1866Tn5252 relaxaseRelaxase, MOBP Family
5-4046..4411 [-], 366Tn5252 ORF9 protein
6-4418..4780 [-], 363Tn5252 ORF10 protein
7-5102..7609 [-], 2508hypothetical protein
8-7707..8411 [-], 705hypothetical protein
9-8411..8917 [-], 507hypothetical protein
10-8957..9313 [-], 357ATPases with chaperone activity, ATP-binding subunit
11-9303..9692 [-], 390Methyl-accepting chemotaxis protein
12-9689..9916 [-], 228hypothetical protein
13-9970..11046 [-], 1077DNA primase
14-11086..11724 [-], 639hypothetical protein
15-11820..12110 [-], 291hypothetical protein
16-12682..12873 [-], 192hypothetical protein
17erm(B)12818..13555 [-], 738MLS methylase
18-13843..14022 [+], 180hypothetical protein
19-14390..14695 [+], 306hypothetical protein
20-14803..15303 [-], 501hypothetical protein
21-15378..15653 [-], 276hypothetical protein
22-15758..16123 [-], 366hypothetical protein
23aphA16606..17400 [-], 795aminoglycoside 3'-phosphotransferase
24-17748..18476 [-], 729streptomycin 3'-adenylyltransferase
25-18689..19216 [-], 528putative adenine phosphoribosyltransferase
26-19260..20105 [-], 846streptomycin adenylyltransferase
27-20204..20602 [-], 399hypothetical protein
28-20818..20985 [-], 168transcriptional regulator Cro/CI family
29tet(40)21864..23084 [-], 1221tetracycline resistance protein, efflux pump
30tet(O/W/32/O)23135..25054 [-], 1920ribosomal protection tetracycline resistance protein
31-25181..25348 [+], 168hypothetical protein
32-25421..25795 [-], 375tnpV protein
33-25735..26466 [-], 732hypothetical protein
34-26561..26851 [-], 291hypothetical protein
35-26865..27164 [-], 300hypothetical protein
36-27235..34059 [-], 6825SNF2 family protein
37-34109..34660 [-], 552hypothetical protein
38-34644..34835 [-], 192hypothetical protein
39-34836..39722 [-], 4887agglutinin receptor precursorPrgB, T4SS component 
40-40003..40593 [+], 591abortive infection protein AbiGI
41-40590..41324 [+], 735hypothetical protein
42-41496..44297 [-], 2802CHAP domain containing proteinPrgK, T4SS component 
43-44299..46629 [-], 2331type IV secretory pathway, VirB4 componentPrgJ, T4SS component 
44-46607..46960 [-], 354hypothetical protein
45-47022..47876 [-], 855Tn5252 ORF23
46-47895..48149 [-], 255hypothetical protein
47-48155..49972 [-], 1818type IV secretory pathway, VirD4 componentT4CP 
48-49972..50460 [-], 489hypothetical protein
49-50538..51122 [-], 585CAAX amino terminal protease family protein
50-51125..51358 [-], 234hypothetical protein
51-51367..51750 [-], 384hypothetical protein
52-51761..52189 [-], 429hypothetical protein
53-52173..53528 [-], 1356C-5 cytosine-specific DNA methylase
54-53677..54495 [-], 819replication initiator protein A (RepA) N-terminus
55-54492..54665 [-], 174hypothetical protein
 
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins55Fasta
(1) Palmieri C; Magi G; Mingoia M; Bagnarelli P; Ripa S; Varaldo PE; Facinelli B (2012). Characterization of a Streptococcus suis tet(O/W/32/O)-carrying element transferable to major streptococcal pathogens. Antimicrob Agents Chemother. 56(9):4697-702. [PudMed:22710115] experimental
(2) Palmieri C; Varaldo PE; Facinelli B (2011). Streptococcus suis, an Emerging Drug-Resistant Animal and Human Pathogen. Front Microbiol. 0.246527778. [PudMed:22275909]
 
experimental experimental literature