ICEberg
I. Information of ICE
ICEberg ID400
Name CTn4 This is a predicted ICE derived from literature
FamilyTn916
OrganismClostridium difficile 630
Size (bp)29975
GC content [Genome] (%)46.68
Insertion site-
Function-
Species that ICE can be transferred to-
Nucleotide SequenceAM180355 (complete ICE sequence in this genome)
Replicon chromosome (4290252 bp, BioProject:78) [NC_009089]
Genome coordinates1284507..1314481 
Putative oriT region -
Putative relaxase coordinates: 1293051..1294400; Locus tag: CD630_11020;  Family:  MOBP


II. ICE interaction with IME/CIME/

The interaction information of CTn4 is not available.



The graph information of CTn4 components from AM180355
Complete gene list of CTn4 from AM180355
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1zupT1280584..1281387 [+], 804putative zinc/iron transporter, ZupT subfamiliy
2CD630_108801281482..1282042 [+], 561conserved hypothetical protein
3rgbR1282293..1283006 [+], 714Two-component response regulator VirR-like
4rgbS1283000..1284385 [+], 1386Fragment of two-component sensor histidine kinase (N-terminal region)
5int1284507..1285700 [-], 1194Integrase Tn1549-like, CTn4-Orf34Integrase 
6xis1285780..1285983 [-], 204Excisionase Tn1549-like, CTn4-Orf33
7CD630_109211286405..1286662 [-], 258putative conjugative transposon protein Tn1549-like, CTn4-Orf32
8CD630_109401286643..1287083 [-], 441putative RNA polymerase sigma factor Tn1549-like, CTn4-Orf31
9CD630_109501287556..1288329 [-], 774ABC-type transport system, multidrug-family permease Tn1549-like, CTn4-Orf30
10CD630_109601288335..1289144 [-], 810ABC-type transport system, multidrug-family permease Tn1549-like, CTn4-Orf29
11CD630_109701289137..1290063 [-], 927ABC-type transport system, multidrug-family ATP-binding protein Tn1549-like, CTn4-Orf28
12CD630_109801290200..1291117 [-], 918Two-component sensor histidine kinase,Tn1549-like, CTn4-Orf27
13CD630_109901291124..1291816 [-], 693Two-component response regulator, Tn1549-like,CTn4-Orf26
14CD630_109911291809..1292030 [-], 222putative conjugative transposon protein Tn1549-like, CTn4-Orf25
15CD630_110001292159..1292512 [+], 354Transcriptional regulator, HTH-type Tn1549-like, CTn4-Orf24
16CD630_110101292761..1293090 [+], 330putative mobilization protein Tn1549-like,CTn4-Orf23
17CD630_110201293051..1294400 [+], 1350putative endonuclease relaxase Tn1549-like,CTn4-Orf22Relaxase, MOBP Family
18CD630_110301294493..1294723 [+], 231putative conjugative transposon protein Tn1549-like, CTn4-Orf21
19CD630_110311294745..1294924 [+], 180putative conjugative transposon protein Tn1549-like, CTn4-Orf20
20CD630_110401295107..1295526 [+], 420putative conjugative transposon protein Tn1549-like, CTn4-Orf19
21CD630_110411295704..1296069 [-], 366putative conjugative transposon protein Tn1549-like, CTn4-Orf18
22CD630_110421296066..1296272 [-], 207putative conjugative transposon protein Tn1549-like, CTn4-Orf17
23CD630_110501296275..1300306 [-], 4032putative antirestriction protein Tn1549-like,CTn4-Orf16
24topB1300439..1302529 [-], 2091DNA topoisomerase III; Tn1549-like, CTn4-Orf15
25CD630_110611302546..1302734 [-], 189putative conjugative transposon protein Tn1549-like, CTn4-Orf14
26CD630_110701302731..1303465 [-], 735putative cell surface protein Tn1549-like,CTn4-Orf13
27CD630_110711303449..1303706 [-], 258putative conjugative transposon protein Tn1549-like, CTn4-Orf12
28CD630_110801303738..1305678 [-], 1941putative conjugative transposon protein Tn1549-like, CTn4-Orf11YddH, T4SS component 
29CD630_110901305705..1306664 [-], 960DNA-methyltransferase, cytosine-N4-specific; Tn1549-like, CTn4-Orf10
30CD630_111001306661..1309090 [-], 2430putative hydrolase Tn1549-like, CTn4-Orf9PrgJ, T4SS component 
31CD630_111101309038..1309442 [-], 405putative conjugative transposon protein Tn1549-like, CTn4-Orf8
32CD630_111201309464..1310333 [-], 870putative membrane protein Tn1549-like,CTn4-Orf7
33CD630_111301310350..1310565 [-], 216putative conjugative transposon protein Tn1549-like, CTn4-Orf6
34CD630_111401310689..1310808 [-], 120putative conjugative transposon protein Tn1549-like, CTn4-Orf5
35CD630_111501310925..1312712 [-], 1788putative conjugative transfer protein Tn1549-like, CTn4-Orf4T4CP 
36CD630_111601312709..1313185 [-], 477putative conjugative transposon protein Tn1549-like, CTn4-Orf3
37CD630_111701313268..1313486 [-], 219putative conjugative transposon protein Tn1549-like, CTn4-Orf2
38CD630_111801313507..1314481 [-], 975putative conjugative transposon protein Tn1549-like, CTn4-Orf1
39CD630_111901315171..1315860 [+], 690putative lipoprotein
40pflD1316081..1318453 [+], 2373Formate acetyltransferase (Pyruvate formate-lyase)
41pflC1318468..1319370 [+], 903Pyruvate formate-lyase 2-activating enzyme
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins34Fasta
(1) Sebaihia M; Wren BW; Mullany P; Fairweather NF; Minton N; Stabler R; Thomson NR; Roberts AP; Cerdeno-Tarraga AM; Wang H; Holden MT; Wright A; Churcher C; Quail MA; Baker S; Bason N; Brooks K; Chillingworth T; Cronin A; Davis P; Dowd L; Fraser A; Feltwell T; Hance Z; Holroyd S; Jagels K; Moule S; Mungall K; Price C; Rabbinowitsch E; Sharp S; Simmonds M; Stevens K; Unwin L; Whithead S; Dupuy B; Dougan G; Barrell B; Parkhill J (2006). The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat Genet. 38(7):779-86. [PudMed:16804543]