ICEberg
I. Information of ICE
ICEberg ID338
Name CMGI-3 This is a predicted ICE derived from literature
FamilyTn4371
OrganismCupriavidus metallidurans CH34
Size (bp)97043
GC content [Genome] (%)64[63.82]
Insertion siteGTAAAA
Function-
Species that ICE can be transferred to-
Nucleotide SequenceCP000352 (complete ICE sequence in this genome)
Replicon chromosome 1 (3928089 bp, BioProject:250) [NC_007973]
Genome coordinates1585489..1682531 
Putative oriT region -
Putative relaxase -


II. ICE interaction with IME/CIME/

The interaction information of CMGI-3 is not available.



The graph information of CMGI-3 components from CP000352
Complete gene list of CMGI-3 from CP000352
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1guaB1581506..1582969 [+], 1464inosine-5'-monophosphate dehydrogenase
2Rmet_14621583057..1583698 [+], 642putative membrane protein
3guaA1583673..1585292 [+], 1620GMP synthetase (glutamine aminotransferase)
4int1585594..1586850 [+], 1257tyrosine-based site-specific recombinaseIntegrase 
5Rmet_14661587051..1587326 [+], 276conserved hypothetical protein
6Rmet_14671587331..1587855 [+], 525conserved hypothetical protein
7Rmet_14681587871..1588281 [-], 411Phage-related protein (modular protein)
8Rmet_14691588375..1589124 [-], 750TatD-related deoxyribonuclease
9Rmet_14701589138..1590505 [-], 1368conserved hypothetical protein
10Rmet_14711590502..1591383 [-], 882conserved hypothetical protein
11Rmet_14721591383..1593317 [-], 1935KAP P-loop
12Rmet_14731593336..1594307 [-], 972putative RNA-directed DNA polymerase (Reverse transcriptase)
13Rmet_64501594273..1594578 [-], 306putative transcriptional regulator (helix-turn-helix motif, XRE-like)
14Rmet_14741594631..1594870 [-], 240hypothetical protein
15Rmet_14751595652..1596002 [+], 351lipoprotein
16Rmet_14761596102..1596440 [-], 339hypothetical protein
17yupB1596748..1597578 [+], 831F plasmid protein 32-like protein (protein belongs to CMGI-3)
18parB1597643..1599685 [+], 2043ParB nuclease
19Rmet_14791599888..1600910 [-], 1023conserved hypothetical protein
20Rmet_14801601776..1602090 [+], 315conserved hypothetical proteinTraF, T4SS component 
21ydcN1602448..1602741 [-], 294putative DNA-binding transcriptional regulator
22Rmet_14821602957..1603304 [+], 348lipoprotein
23Rmet_14831603648..1604439 [+], 792conserved hypothetical protein
24Rmet_14841604478..1604804 [+], 327putative transcriptional regulator (DNA binding protein)
25repA1604819..1605688 [+], 870replication initiation protein CMGI-3 (GI family Tn4371)
26parA1605942..1606580 [+], 639ATPase involved in plasmid partitioning ParA
27parG1606577..1606861 [+], 285regulator of plasmid copy number (ribbon-helix-helix)
28Rmet_14871606858..1607400 [+], 543conserved hypothetical protein
29traF1607397..1607984 [+], 588plasmid conjugal transfer transmembrane proteinTraF, T4SS component 
30virD21608307..1610409 [+], 2103Relaxase type IV secretory pathway VirD2
31Rmet_14901610418..1611011 [-], 594putative transcriptional regulator (fragment)
32tnpA1611195..1614110 [+], 2916transposase IS1071
33cbbA11614223..1615083 [-], 861Fructose-bisphosphate aldolase 1 (Fba)
34Rmet_14931615177..1615350 [-], 174-
35cbbY (delta)1615529..1615732 [-], 204-
36cbbY (delta)1615745..1615948 [-], 204-
37Rmet_14961615994..1616368 [-], 375hypothetical protein
38Rmet_14971616384..1616680 [-], 297conserved hypothetical protein
39cbbO1616689..1619046 [-], 2358Rubisco activation protein
40cbbQ1619059..1619862 [-], 804Rubisco activation protein
41cbbS1620012..1620368 [-], 357ribulose 1,5-bisphosphate carboxylase/oxygenase, RuBisCO (type I) small subunit (CbbS)
42cbbL1620446..1621867 [-], 1422ribulose 1,5-bisphosphate carboxylase/oxygenase, RuBisCo (type I) large subunit (CbbL)
43cbbR11621992..1622873 [+], 882RuBisCO operon transcriptional regulator
44Rmet_64521622912..1623031 [+], 120hypothetical protein
45tnpA1623052..1624302 [+], 1251transposase IS1090
46Rmet_15041624306..1624614 [-], 309-
47Rmet_15051624713..1625294 [-], 582-
48tnpA1625591..1628506 [-], 2916transposase IS1071
49cbbF11628605..1629444 [-], 840fructose-1,6-bisphosphatase
50Rmet_64531629650..1629802 [-], 153hypothetical protein
51cbbR21629952..1630875 [-], 924LysR-type transcriptional regulator (RuBisCO operon transcriptional regulator)
52cbbS(delta)1630981..1631145 [+], 165-
53cbbE11631163..1631861 [+], 699D-ribulose-5-phosphate 3-epimerase
54cbbF21632041..1633138 [+], 1098fructose-1,6-bisphosphatase I
55cbbP1633171..1634055 [+], 885phosphoribulokinase
56cbbT11634055..1636109 [+], 2055transketolase 1, thiamin-binding protein
57cbbZ11636111..1636842 [+], 732phosphoglycolate phosphatase (PGP)
58cbbG11636839..1637846 [+], 1008glyceraldehyde-3-phosphate dehydrogenase A (GAPDH-A)
59pgk11637843..1639033 [+], 1191phosphoglycerate kinase (Pgk)
60cbbY1639030..1639848 [+], 819CbbY, protein of unknown function linked to the Calvin-Benson-Bassham cycle, HAD-like hydrolase
61cbbA21639908..1640945 [+], 1038Fructose-bisphosphate aldolase (Fba)
62pykA21640960..1642378 [+], 1419pyruvate kinase
63cbbJ11642414..1643232 [+], 819triosephosphate isomerase (CbbJ)
64cbbI11643232..1643891 [+], 660ribose 5-phosphate isomerase (cbbI)
65hoxF1644017..1645825 [+], 1809NAD-reducing hydrogenase diaphorase moiety large subunit
66hoxU1645822..1646532 [+], 711NAD-reducing hydrogenase diaphorase moiety small subunit
67hoxY1646529..1647158 [+], 630NAD-reducing hydrogenase hoxS delta subunit
68hoxH1647176..1648642 [+], 1467NAD-reducing hydrogenase hoxS subunit beta
69hoxW1648626..1649132 [+], 507((NiFe) hydrogenase C-terminal protease)
70hoxI1649145..1649648 [+], 504cyclic nucleotide-binding domain (cNMP-BD) protein
71pntAA'1649985..1650716 [+], 732-
72tnpA1650747..1651775 [-], 1029transposase IS1088
73pntAA'1651820..1652236 [+], 417-
74pntAB1652237..1652542 [+], 306-
75pntB1652539..1653912 [+], 1374NAD(P) transhydrogenase subunit beta
76hoxN1653974..1654810 [+], 837High-affinity nickel-transporter
77Rmet_64541655015..1655209 [+], 195hypothetical protein
78Rmet_15341655263..1655568 [+], 306-
79hypA21655561..1655902 [+], 342Hydrogenase nickel incorporation protein hypA (Protein hupA)
80hypB21655936..1656916 [+], 981GTP hydrolase involved in nickel liganding into hydrogenases
81hypF21656930..1659287 [+], 2358carbamoyl phosphate phosphatase and maturation protein for (NiFe) hydrogenases
82hypC21659340..1659528 [+], 189Hydrogenase expression/formation protein HypC
83hypD21659525..1660667 [+], 1143protein required for maturation of hydrogenases
84hypE21660741..1661790 [+], 1050carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein
85hoxX1661889..1663628 [+], 1740Hydrogenase maturation factor hoxX
86hoxA1663625..1664554 [+], 930Hydrogenase transcriptional regulatory protein
87Rmet_64551663967..1664401 [-], 435hypothetical protein
88cbbA (delta)1664544..1664927 [-], 384-
89tnpA1665103..1668018 [+], 2916transposase IS1071
90Rmet_15451668181..1668588 [-], 408hypothetical protein
91traR1668624..1669604 [+], 981Transcriptional regulator, LysR family (CMGI-3)
92Rmet_15471669601..1669879 [+], 279lipoprotein precursor (transfer module of CMGI-3)
93traG1670131..1672125 [+], 1995conjugal transfer proteinVirD4, T4SS component 
94Rmet_15491672122..1672586 [+], 465DNA-binding helix-turn-helix domain-containing protein (CopG-like) (belongs to the transfer module of genomic island CMGI-3)
95trbB1672583..1673659 [+], 1077P-type conjugative transfer ATPase TrbB (CMGI-2)TrbB, T4SS component 
96trbC1673656..1674039 [+], 384mating pair formation conjugal transfer TrbC transmembrane protein (module transfer of genomic island CMGI-3)TrbC, T4SS component 
97trbD1674036..1674308 [+], 273putative conjugal transfer transmembrane proteinTrbD, T4SS component 
98trbE1674324..1676753 [+], 2430ATPase involved in conjugal transfer CagE TrbE VirB component of type IV transporter system (belongs to transfer module of genomic island CMGI-3)TrbE, T4SS component 
99trbJ1676750..1677475 [+], 726mating pair formation proteinTrbJ, T4SS component 
100Rmet_15551677488..1677820 [+], 333lipoproteinTrbJ, T4SS component 
101trbL1677817..1679202 [+], 1386conjugal transfer transmembrane proteinTrbL, T4SS component 
102trbF1679215..1679919 [+], 705conjugal transfer precursor proteinTrbF, T4SS component 
103trbG1679916..1680917 [+], 1002Conjugal transfer protein precursorTrbG, T4SS component 
104trbI1680920..1682188 [+], 1269type IV secretion system transmembrane protein (conjugal transfer:mating pair formation )TrbI, T4SS component 
105Rmet_15601682185..1682445 [+], 261conserved hypothetical protein
106Rmet_15611682540..1683277 [+], 738conserved hypothetical protein
107Rmet_15621683274..1684194 [+], 921conserved hypothetical protein
108tnpA1684483..1685010 [+], 528transposase DNA binding site ISRme12
109tnpB1685010..1685846 [+], 837transposase catalytic site ISRme12
110Rmet_15651685944..1687476 [-], 1533Flavin-containing monooxygenase FMO:IMP dehydrogenase/GMP reductase:FAD dependent oxidoreductase
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins91Fasta
(1) Janssen PJ; Van Houdt R; Moors H; Monsieurs P; Morin N; Michaux A; Benotmane MA; Leys N; Vallaeys T; Lapidus A; Monchy S; Medigue C; Taghavi S; McCorkle S; Dunn J; van der Lelie D; Mergeay M (2010). The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments. PLoS One. 5(5):e10433. [PudMed:20463976]