ICEberg
I. Information of ICE
ICEberg ID337
Name CMGI-2 This is a predicted ICE derived from literature
FamilyTn4371
OrganismCupriavidus metallidurans CH34
Size (bp)101638
GC content [Genome] (%)63.15[63.82]
Insertion siteTTTTTGTTT
Function-
Species that ICE can be transferred to-
Nucleotide SequenceCP000352 (complete ICE sequence in this genome)
Replicon chromosome 1 (3928089 bp, BioProject:250) [NC_007973]
Genome coordinates1359263..1460900 
Putative oriT region -
Putative relaxase coordinates: 1379309..1381414; Locus tag: Rmet_1259;  Family:  MOBL


II. ICE interaction with IME/CIME/

The interaction information of CMGI-2 is not available.



The graph information of CMGI-2 components from CP000352
Complete gene list of CMGI-2 from CP000352
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1Rmet_12331355701..1356477 [+], 777hypothetical protein
2Rmet_12341356502..1356738 [+], 237conserved hypothetical protein DUF465
3yoaA1356779..1359010 [+], 2232putative ATP-dependent helicase
4int1359445..1360644 [+], 1200tyrosine-based site-specific recombinase CMGI-2
5Rmet_12371360641..1361717 [+], 1077conserved hypothetical protein (secreted)
6radC'1362069..1362485 [+], 417-
7ritA21362583..1363842 [+], 1260tyrosine-based site-specific recombinase
8ritB21363839..1364831 [+], 993tyrosine-based site-specific recombinase
9ritC21364828..1365838 [+], 1011tyrosine-based site-specific recombinase , N-terminal SAM-like proteinIntegrase 
10radC'1366076..1366186 [+], 111-
11Rmet_12431366465..1366857 [+], 393conserved hypothetical protein
12yupB1367383..1368390 [+], 1008F plasmid protein 32-like protein (CMGI-2)
13parB1368469..1370520 [+], 2052nuclease ParB (CMGI-2)
14Rmet_12461370728..1371246 [-], 519GCN5-related N-acetyltransferase, GNAT family
15Rmet_12471371398..1371727 [+], 330-
16Rmet_64451371609..1371872 [-], 264hypothetical protein
17Rmet_12481372035..1372388 [+], 354conserved hypothetical proteinTraF, T4SS component 
18Rmet_12491372453..1372758 [-], 306transcription regulator (helix turn helix motif) XRE family
19Rmet_12501372771..1373352 [+], 582putative lipoprotein
20tnp1373579..1374580 [-], 1002transposase ISRme5 (copy a, CMGI-2)
21Rmet_12521374596..1375468 [+], 873conserved hypothetical protein
22Rmet_12531375516..1375833 [+], 318Putative dna binding protein
23repA1375860..1376717 [+], 858replication initiator and transcription repressor protein
24parA1376971..1377609 [+], 639ATPase involved in plasmid partitioning
25parG1377606..1377878 [+], 273regulator of plasmid copy number
26Rmet_12571377875..1378408 [+], 534conserved hypothetical protein; putative exported protein
27traF1378405..1378992 [+], 588plasmid conjugal transfer transmembrane proteinTraF, T4SS component 
28virD21379309..1381414 [+], 2106Relaxase type IV secretory pathway VirD2(protein belongs to CMGI-2)Relaxase, MOBL Family
29Rmet_12601381452..1381913 [-], 462conserved hypothetical protein; putative exported protein
30Rmet_12611381974..1383101 [-], 1128putative secreted protein (protein belongs to CMGI-2)
31tnpA1383234..1384484 [-], 1251transposase IS1090
32tnpB1384634..1385440 [-], 807IstB-like ATP binding protein ISRme20TrbB, T4SS component 
33tnpA1385440..1386450 [-], 1011transposase (DDE site) ISRme20
34Rmet_12651386618..1387484 [-], 867conserved hypothetical protein
35Rmet_12661387484..1388893 [-], 1410putative RNA Modifying Enzyme
36Rmet_64461388901..1389104 [-], 204conserved hypothetical protein
37oprN1389124..1390662 [-], 1539Outer membrane efflux family protein precursor
38ybhR1390659..1391777 [-], 1119permease component of ABC superfamily (CMGI-2)
39ybhS1391782..1392951 [-], 1170putative transporter subunit: permease component of ABC superfamily
40Rmet_12701393084..1393290 [-], 207-
41ritA11393469..1394701 [+], 1233tyrosine-based site-specific recombinase (recombinase triad in CMGI-2)
42ritB11394698..1395645 [+], 948tyrosine based site-specific recombinase
43ritC11395642..1396637 [+], 996tyrosine-based site-specific recombinase
44tnpA1396731..1398221 [-], 1491-
45Rmet_12751398244..1398603 [-], 360conserved hypothetical protein
46Rmet_12761398603..1398938 [-], 336conserved hypothetical protein
47Rmet_12771398971..1399222 [-], 252putative transporter fused subunits of ABC superfamily: ATP-binding components (fragment)
48Rmet_12781399238..1399627 [+], 390-
49Rmet_12791399619..1399882 [-], 264-
50tnpA1400002..1401393 [+], 1392transposase ISRme5 (copy b, CMGI-2)
51hypE11401177..1402289 [-], 1113carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (CMGI-2)
52hypD11402286..1403449 [-], 1164protein required for maturation of hydrogenases (CMGI-2)
53hypC11403458..1403685 [-], 228HypC1 hydrogenase 2 accessory protein (CMGI-2)
54hypF11403676..1404815 [-], 1140HypF1 hydrogenase maturation protein (CMGI-2)
55hypB11404829..1405866 [-], 1038HypB1 GTP hydrolase involved in nickel liganding into hydrogenases (CMGI-2)
56hypA11405890..1406237 [-], 348HypA1 Hydrogenase nickel incorporation protein hypA (CMGI-2)
57hoxV1406230..1407384 [-], 1155HoxV Hydrogenase expression/formation protein hoxV (CMGI-2)
58hoxT1407413..1407889 [-], 477HoxT putative hydrogenase expression/formation protein (CMGI-2)
59hoxR1407891..1408121 [-], 231HoxR Rubredoxin (Rd)Electron transport; (belongs to CMGI-2)
60hoxQ1408127..1408990 [-], 864HoxQ protein involved in nickel incorporation into hydrogenase-1 proteins(belongs to CMGI-2)
61hoxO1409022..1409471 [-], 450HoxO Hydrogenase expression/formation protein hoxO (belongs to CMGI-2)
62hoxL1409487..1409789 [-], 303HoxL hydrogenase expression/formation protein hoxL (belongs to CMGI-2)
63hoxM1409791..1410456 [-], 666HoxM protein involved in processing of HyaA and HyaB proteins Hydrolase; Nickel Metalloprotease; (belongs to CMGI-2)Protease Absolutely required for hydrogenase activity
64Rmet_12941410478..1410861 [-], 384putative plasmid maintenance system antidote protein, XRE family Putative HTH-type transcriptional regulator ybaQ (belongs to CMGI-2)
65hoxZ1410945..1411676 [-], 732hoxZ Probable membrane-bound Ni/Fe-hydrogenase-linked b-type cytochrome (belongs to CMGI-2)
66hupE1411714..1412451 [-], 738HupE putative membrane-bound hydrogenase component hupE HupE/UreJ protein precursor (modular protein)Uptake hydrogenase (belongs to CMGI-2)
67hoxG1412448..1414307 [-], 1860HoxG hydrogenase 1, large subunit Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound hydrogenase large subunit) (belongs to CMGI-2)
68hoxK1414328..1415422 [-], 1095HoxK membrane-bound (NiFe) hydrogenase small subunit (belongs to CMGI-2)
69Rmet_12991415555..1415956 [+], 402hypothetical protein (belongs to CMGI-2)
70Rmet_13001415972..1416667 [-], 696choline dehydrogenase (CDH) (partial)(belongs to CMGI-2)
71tnpA1416784..1417749 [+], 966transposase ISRme5 (copy c)
72tnpB1417721..1418242 [-], 522-
73tnpA1418275..1418538 [-], 264IS3/IS407-type transposase (orfA)(belongs to CMGI-2) DNA-binding protein second orf of element are fragments Rmet_1302 and Rmet_1279
74Rmet_13041418613..1419044 [+], 432-
75patR1419138..1420982 [-], 1845transcriptional regulatory protein with helix-turn-helix motif, Fis-type (belongs to CMGI-2)
76Rmet_13061421155..1421985 [-], 8312-hydroxymuconic semialdehyde hydrolase (HMSH) - alpha/beta hydrolase superfamily (belongs to CMGI-2)
77Rmet_64471422085..1422249 [-], 165conserved hypothetical protein (partial)(belongs to CMGI-2)
78Rmet_13071422721..1424115 [-], 1395membrane protein precursor involved in aromatic hydrocarbon degradation (TbuX/FadL-like)(belongs to CMGI-2)
79Rmet_13081424194..1425255 [-], 1062Alcohol dehydrogenase GroES-like protein (belongs to CMGI-2)
80tnpA1425425..1425835 [+], 411transposase (orfA) ISRme6
81Rmet_64481425898..1426242 [+], 345putative transposase (orfB) ISRme6
82Rmet_13101426313..1426765 [-], 453-
83Rmet_13111426985..1428007 [-], 1023ferredoxin--NAD(+) reductase (FAD/NAD(P)-binding region)(belongs to CMGI-2)
84tmoE1428107..1429093 [-], 987Toluene-4-monooxygenase system protein E (belongs to CMGI-2)
85tmoD1429148..1429462 [-], 315Toluene-4-monooxygenase system protein D
86tmoC1429503..1429838 [-], 336Toluene-4-monooxygenase system protein C (belongs to CMGI-2)
87tmoB1429894..1430160 [-], 267Toluene-4-monooxygenase system protein B (belongs to CMGI-2)
88tmoA1430229..1431731 [-], 1503Toluene-4-monooxygenase system protein A (belongs to CMGI-2)
89nahJ1431791..1431982 [-], 1924-oxalocrotonate tautomerase family enzyme (protein belongs to CMGI-2)
90dmpH1431994..1432782 [-], 7894-oxalocrotonate decarboxylase (protein belongs to CMGI-2)
91bphI1432779..1433825 [-], 10474-hydroxy-2-oxovalerate/4-hydroxy-2- oxopentanoic acid aldolase, class I 4-Hydroxy-2-oxovalerate aldolase (protein belongs to CMGI-2)
92mhpF1433845..1434756 [-], 912Acetaldehyde-CoA dehydrogenase II, NAD-binding (protein belongs to CMGI-2)
93xylJ1434835..1435623 [-], 7892-hydroxypent-2,4-dienoate hydratase (protein belongs to CMGI-2)
94dmpC1435620..1437131 [-], 15122-hydroxymuconic semialdehyde dehydrogenase (HMSD)(protein belongs to CMGI-2)
95Rmet_13231437198..1437647 [-], 450Hypothetical protein
96dmpB1437669..1438613 [-], 945Metapyrocatechase (MPC) (CatO2ase) (Catechol 2,3- dioxygenase) (C23O) (protein belongs to CMGI-2)
97Rmet_13251438631..1438987 [-], 357Ferredoxin (protein belongs to CMGI-2)
98tomA51438990..1440054 [-], 1065Phenol hydroxylase P5 protein (Phenol 2-monooxygenase P5 component) (protein belongs to CMGI-2)
99tomA41440133..1440489 [-], 357toluene ortho-monooxygenase P4 protein (toluene ortho-monooxygenase P4 component) (protein belongs to CMGI-2)
100tomA31440486..1442045 [-], 1560toluene hydroxylase P3 protein (toluene 2-monooxygenase P3 component) (protein belongs to CMGI-2)
101tomA21442068..1442337 [-], 270phenol hydroxylase P2 protein (phenol 2-monooxygenase P2 component) (protein belongs to CMGI-2)
102tomA11442377..1443372 [-], 996Phenol hydroxylase P1 protein (Phenol 2-monooxygenase P1 component)(protein belongs to CMGI-2)
103tomA01443460..1443684 [-], 225TomA0 (protein belongs to CMGI-2)
104Rmet_13321444219..1444560 [+], 342-
105phaB21444571..1445317 [+], 7473-oxoacyl-(acyl-carrier-protein) reductase (protein belongs to CMGI-2)
106uspA21445377..1445841 [+], 465universal stress protein, UspA family (protein belongs to CMGI-2)
107chnA1446018..1446806 [+], 789Cyclohexanol dehydrogenase (protein belongs to CMGI-2)
108traR1446803..1447765 [+], 963Transcriptional regulator, LysR family (CMGI-2)
109Rmet_13371447762..1448040 [+], 279lipoprotein precursor (transfer module CMGI-2)
110Rmet_13381447986..1448282 [-], 297hypothetical protein
111traG1448292..1450286 [+], 1995Conjugal transfer protein TraG (CMGI-2)VirD4, T4SS component 
112Rmet_13401450283..1450747 [+], 465DNA-binding helix-turn-helix domain-containing protein (CopG-like) (CMGI-2)
113trbB1450744..1451808 [+], 1065P-type conjugative transfer ATPase TrbB (CMGI-2)TrbB, T4SS component 
114trbC1451805..1452188 [+], 384conjugal transfer TrbC transmembrane protein (CMGI-2)TrbC, T4SS component 
115trbD1452185..1452457 [+], 273conjugal transfer TrbD transmembrane protein (protein belongs to CMGI-2)TrbD, T4SS component 
116trbE1452470..1454929 [+], 2460ATPase involved in conjugal transfer CagE TrbE VirB component of type IV transporter system (protein belongs to CMGI-2)TrbE, T4SS component 
117trbJ1454926..1455663 [+], 738conjugal transfer TrbJ signal peptide protein (protein belongs to CMGI-2)TrbJ, T4SS component 
118Rmet_13461455676..1455996 [+], 321lipoprotein (protein belongs to CMGI-2)TrbJ, T4SS component 
119trbL1455993..1457372 [+], 1380conjugal transfer trbL transmembrane protein (protein belongs to CMGI-2)TrbL, T4SS component 
120trbF1457397..1458101 [+], 705conjugal transfer trbF precursor (protein belongs to CMGI-2)TrbF, T4SS component 
121trbG1458098..1459090 [+], 993Conjugal transfer protein TrbG/VirB9/CagX precursor putative exported protein(protein belongs to CMGI-2)TrbG, T4SS component 
122trbI1459093..1460364 [+], 1272type IV secretion system transmembrane protein (conjugal transfer:mating pair formation ) (protein belongs to CMGI-2)TrbI, T4SS component 
123Rmet_13511460361..1460606 [+], 246conserved hypothetical protein CMGI-2
124comL1460941..1461777 [-], 837DNA uptake lipoprotein
125rluD1461789..1463000 [+], 121223S rRNA pseudouridine synthase
126Rmet_13541462987..1463778 [+], 792conserved hypothetical protein
127Rmet_13551463804..1464031 [-], 228putative transmembrane protein
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins109Fasta
(1) Janssen PJ; Van Houdt R; Moors H; Monsieurs P; Morin N; Michaux A; Benotmane MA; Leys N; Vallaeys T; Lapidus A; Monchy S; Medigue C; Taghavi S; McCorkle S; Dunn J; van der Lelie D; Mergeay M (2010). The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments. PLoS One. 5(5):e10433. [PudMed:20463976]