ICEberg
I. Information of ICE
ICEberg ID191
Name 10270-RD.2 This is a predicted ICE derived from literature
Family10270-RD.2
OrganismStreptococcus pyogenes MGAS10270
Size (bp)34300
GC content [Genome] (%)34.63[38.43]
Insertion site-
FunctionVirulence: encodes two cell surface anchored adhesins; Spy1399 and Spy1410.
Species that ICE can be transferred to-
Nucleotide SequenceCP000260 (complete ICE sequence in this genome)
Replicon chromosome (1928252 bp, BioProject:16364) [NC_008022]
Genome coordinates1312338..1346637 
Putative oriT region -
Putative relaxase coordinates: 1326499..1327740; Locus tag: MGAS10270_Spy1395;  Family:  MOBT


II. ICE interaction with IME/CIME/

The interaction information of 10270-RD.2 is not available.



The graph information of 10270-RD.2 components from CP000260
Complete gene list of 10270-RD.2 from CP000260
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1valS1307544..1310192 [-], 2649Valyl-tRNA synthetase
2MGAS10270_Spy13741310194..1310778 [-], 585hypothetical protein
3MGAS10270_Spy13751310754..1310933 [-], 180Ribosomal-protein-serineacetyltransferase
4MGAS10270_Spy13761311358..1311753 [-], 396hypothetical cytosolic protein
5MGAS10270_Spy13771311771..1312025 [-], 255hypothetical protein
6int31312338..1313474 [-], 1137DNA integration/recombination/inversion proteinIntegrase 
7MGAS10270_Spy13791313524..1313856 [-], 333hypothetical protein
8MGAS10270_Spy13801314023..1314565 [-], 543hypothetical membrane spanning protein
9MGAS10270_Spy13811314788..1315549 [-], 762Cell surface proteinPrgC, T4SS component 
10MGAS10270_Spy13821315574..1316131 [-], 558hypothetical exported protein
11MGAS10270_Spy13831316131..1317489 [-], 1359hypothetical exported protein
12MGAS10270_Spy13841317624..1317767 [+], 144hypothetical protein
13MGAS10270_Spy13851317935..1320250 [-], 2316Membrane proteinYddG, T4SS component 
14MGAS10270_Spy13861320265..1322766 [-], 2502DNA segregation ATPaseYddE, T4SS component 
15MGAS10270_Spy13871322787..1323197 [-], 411hypothetical proteinYddD, T4SS component 
16MGAS10270_Spy13881323200..1323469 [-], 270hypothetical membrane spanning protein
17MGAS10270_Spy13891323488..1324438 [-], 951hypothetical proteinYddB, T4SS component 
18MGAS10270_Spy13901324439..1324918 [-], 480hypothetical protein
19MGAS10270_Spy13911324926..1325177 [-], 252hypothetical protein
20MGAS10270_Spy13921325207..1325416 [-], 210hypothetical protein
21MGAS10270_Spy13931325460..1325732 [-], 273hypothetical protein
22MGAS10270_Spy13941326121..1326498 [-], 378hypothetical cytosolic protein
23MGAS10270_Spy13951326499..1327740 [-], 1242Transcriptional regulator, Cro/CI familyRelaxase, MOBT Family
24MGAS10270_Spy13961328052..1329761 [-], 1710FtsK/SpoIIIE familyYdcQ, T4SS component 
25MGAS10270_Spy13971329781..1330236 [-], 456hypothetical cytosolic protein
26MGAS10270_Spy13981330239..1330544 [-], 306hypothetical cytosolic protein
27MGAS10270_Spy13991330806..1334864 [-], 4059Cell surface proteinPrgB, T4SS component 
28MGAS10270_Spy14001335034..1337274 [-], 2241M proteinPrgA, T4SS component 
29MGAS10270_Spy14011337367..1337759 [-], 393hypothetical cytosolic protein
30MGAS10270_Spy14021337883..1338323 [-], 441Glutaredoxin-like protein
31MGAS10270_Spy14031338481..1338915 [-], 435Transcriptional regulator, Cro/CI family
32MGAS10270_Spy14041339790..1340176 [+], 387Transcriptional regulator, Cro/CI family
33MGAS10270_Spy14051340183..1340605 [+], 423hypothetical cytosolic protein
34MGAS10270_Spy14061340670..1341026 [-], 357hypothetical exported protein
35MGAS10270_Spy14071341029..1341367 [-], 339hypothetical cytosolic protein
36MGAS10270_Spy14081341364..1341657 [-], 294DNA-damage-inducible protein J
37MGAS10270_Spy14091341678..1341863 [+], 186Transposase
38MGAS10270_Spy14101342369..1345203 [-], 2835Cell surface protein
39MGAS10270_Spy14111345432..1346637 [+], 1206Transcriptional regulator, AraC family
40MGAS10270_Spy14121347155..1347907 [+], 7533-deoxy-7-phosphoheptulonate synthase
41aroB1347948..1349036 [+], 10893-dehydroquinate synthase
42MGAS10270_Spy14141349306..1349464 [-], 159hypothetical protein
43MGAS10270_Spy14151349472..1349777 [-], 306Putative acetate kinase
44MGAS10270_Spy14161349779..1350117 [-], 339hypothetical cytosolic protein
45MGAS10270_Spy14171350170..1350925 [-], 756SAM-dependent methyltransferase
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins34Fasta
(1) Sitkiewicz I; Green NM; Guo N; Mereghetti L; Musser JM (2011). Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins. BMC Microbiol. 0.503472222. [PudMed:21457552] experimental
(2) Beres SB; Musser JM (2007). Contribution of exogenous genetic elements to the group A Streptococcus metagenome. PLoS One. 2(8):e800. [PudMed:17726530]
 
experimental experimental literature