ICEberg
I. Information of ICE
ICEberg ID181
Name 10750-RD.2 This is a predicted ICE derived from literature
FamilyTn1806
OrganismStreptococcus pyogenes MGAS10750
Size (bp)48521
GC content [Genome] (%)31.25
Insertion site-
FunctionType II restriction-modification system; spectinomycin resistance; erythromycin Resistance
Species that ICE can be transferred to-
Nucleotide SequenceCP000262 (complete ICE sequence in this genome)
Replicon chromosome (1937111 bp, BioProject:16366) [NC_008024]
Genome coordinates1621728..1670248 
Putative oriT region -
Putative relaxase coordinates: 1659807..1660178; Locus tag: MGAS10750_Spy1710;  Family:  MOBP


II. ICE interaction with IME/CIME/

The interaction information of 10750-RD.2 is not available.



The graph information of 10750-RD.2 components from CP000262
Complete gene list of 10750-RD.2 from CP000262
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1hsdS1619589..1620764 [+], 1176Type I restriction-modification system specificity subunit
2MGAS10750_Spy16781620777..1621568 [+], 792Type I restriction-modification system methylation subunit
3MGAS10750_Spy16791621728..1622501 [+], 774phage protein
4MGAS10750_Spy16801622498..1623325 [+], 828Replicative DNA helicase
5MGAS10750_Spy16811623318..1623803 [+], 486hypothetical protein
6MGAS10750_Spy16821623800..1624534 [+], 735phage antirepressor protein
7MGAS10750_Spy16831624512..1626323 [+], 1812TraG/TraD family
8MGAS10750_Spy16841626342..1626617 [+], 276hypothetical protein
9MGAS10750_Spy16851626808..1627119 [+], 312hypothetical protein
10MGAS10750_Spy16861627121..1627336 [+], 216hypothetical protein
11MGAS10750_Spy16871627347..1628210 [+], 864hypothetical protein
12MGAS10750_Spy16881628220..1628510 [+], 291hypothetical protein
13MGAS10750_Spy16891628512..1628922 [+], 411hypothetical protein
14MGAS10750_Spy16901628813..1630831 [+], 2019TrsET4CP 
15MGAS10750_Spy16911630788..1631237 [+], 450TrsEPrgJ, T4SS component 
16MGAS10750_Spy16921631245..1633824 [+], 2580NlpC/P60 family proteinOrf14_Tn, T4SS component 
17MGAS10750_Spy16931633839..1634078 [+], 240hypothetical protein
18MGAS10750_Spy16941634065..1636416 [+], 2352Putative surface protein
19MGAS10750_Spy16951636431..1637300 [+], 870hypothetical protein
20MGAS10750_Spy16961637352..1639085 [+], 1734DNA topoisomerase III
21MGAS10750_Spy16971639078..1640043 [+], 966Type II restriction-modification system methylation subunit
22MGAS10750_Spy16981640030..1647673 [+], 7644Superfamily II DNA and RNA helicase
23MGAS10750_Spy16991647717..1648373 [+], 657hypothetical protein
24MGAS10750_Spy17001648925..1649557 [+], 633Transcriptional regulator, TetR family
25MGAS10750_Spy17011649642..1650523 [+], 882Tetronasin resistance ATP-binding protein
26MGAS10750_Spy17021650456..1652162 [+], 1707Tetronasin resistance transmembrane protein
27MGAS10750_Spy17041652743..1652949 [-], 207hypothetical protein
28MGAS10750_Spy17031652896..1653627 [+], 732rRNA adenine N-6-methyltransferase
29MGAS10750_Spy17051653844..1654368 [+], 525spectinomycin phosphotransferase
30MGAS10750_Spy17061654414..1654803 [+], 390Cytidine deaminase
31MGAS10750_Spy17071654782..1655486 [+], 705Zn-dependent hydrolase
32MGAS10750_Spy17081656371..1657465 [-], 1095Relaxase
33MGAS10750_Spy17091657466..1659343 [-], 1878Reverse transcriptase / RNA maturase / Endonuclease
34MGAS10750_Spy17101659807..1660178 [-], 372RelaxaseRelaxase, MOBP Family
35MGAS10750_Spy17111660180..1660638 [-], 459hypothetical protein
36MGAS10750_Spy17121660707..1661801 [-], 1095RhuM
37MGAS10750_Spy17131662273..1662824 [-], 552phage-related protein
38MGAS10750_Spy17141662833..1663234 [-], 402Transcriptional regulator, Cro/CI family
39MGAS10750_Spy17151663384..1663944 [+], 561hypothetical protein
40MGAS10750_Spy17161664213..1664623 [+], 411hypothetical protein
41MGAS10750_Spy17171665419..1667092 [+], 1674Site-specific recombinase
42MGAS10750_Spy17181667092..1668762 [+], 1671Site-specific recombinase
43MGAS10750_Spy17191668755..1670248 [+], 1494Site-specific recombinaseIntegrase 
44hsdM1670421..1671224 [+], 804Type I restriction-modification system methylation subunit
45MGAS10750_Spy17211671605..1671724 [+], 120hypothetical protein
46salR1671781..1672386 [-], 606Transcriptional regulatory protein degU
47MGAS10750_Spy17231672367..1673929 [-], 1563Sensory transduction protein kinase
 
flank Flanking regions
integrase Gene may contribute to site-specific recombination
conjugation Gene may play role in conjugative transfer

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins41Fasta
(1) Sitkiewicz I; Green NM; Guo N; Mereghetti L; Musser JM (2011). Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins. BMC Microbiol. 0.503472222. [PudMed:21457552] experimental
(2) Brenciani A; Tiberi E; Bacciaglia A; Petrelli D; Varaldo PE; Giovanetti E (2011). Two distinct genetic elements are responsible for erm(TR)-mediated erythromycin resistance in tetracycline-susceptible and tetracycline-resistant strains of Streptococcus pyogenes. Antimicrob Agents Chemother. 55(5):2106-12. [PudMed:21343455] experimental
(3) Beres SB; Musser JM (2007). Contribution of exogenous genetic elements to the group A Streptococcus metagenome. PLoS One. 2(8):e800. [PudMed:17726530]
 
experimental experimental literature