ICEberg
I. Information of ICE
ICEberg ID122
Name pMEA100 {AICE} This ICE is derived from experimental literature
FamilyUnclassified
OrganismAmycolatopsis mediterranei LBG A3136
Size (bp)23290
GC content [Genome] (%)68.44
Insertion sitetRNA-Phe
FunctionEliciting the lethal zygosis phenotype
Species that ICE can be transferred to-
Nucleotide SequenceEU149765 (complete ICE sequence in this GenBank file)
Replicon-
Coordinates1..23290 
Putative oriT region -
Putative relaxase -


II. ICE interaction with IME/CIME/

The interaction information of pMEA100 {AICE} is not available.



The graph information of pMEA100 {AICE} components from EU149765
Complete gene list of pMEA100 {AICE} from EU149765
#Gene Coordinates [+/-], size (bp) Product 
(GenBank annotation)
*Reannotation 
1repAM1..1113 [+], 1113replication initiator protein
2xis1434..1736 [+], 303excisionase
3int1752..3098 [+], 1347bacteriophage-related integraseIntegrase 
4amp1043394..3855 [+], 462hypothetical protein
5amp1054891..5238 [+], 348hypothetical protein
6amp1065552..6346 [-], 795hypothetical protein
7mdp7116..7688 [+], 573metal dependent phosphohydrolase
8nud17680..8156 [-], 477NUDIX hydrolase
9amp1098162..8911 [-], 750XRE family transcriptional regulator
10amp1109084..9287 [+], 204pMEA-specific protein
11korA9284..9631 [-], 348GntR family transcriptional regulator
12amp1129752..10009 [+], 258hypothetical protein
13amp11310318..11040 [+], 723hypothetical protein
14nud211081..11605 [+], 525NUDIX hydrolase
15ssgA11623..11976 [+], 354SsgA family transcriptional regulator
16traF12445..12675 [+], 231TraF
17traG12675..12989 [+], 315TraG
18traH12880..13314 [+], 435TraH
19traI13311..14492 [+], 1182TraI
20traJ14599..16911 [+], 2313TraJ
21amp12117208..17654 [+], 447hypothetical protein
22amp12217644..18387 [-], 744hypothetical protein
23ssb18442..18924 [+], 483single-strand DNA-binding protein
24amp12419233..20189 [+], 957N-terminal primase-polymerase (primpol)-domain containing protein
25amp12520523..21023 [+], 501hypothetical protein
26amp12621020..21373 [+], 354hypothetical protein
27dmt21373..22998 [+], 1626C-5 cytosine-specific DNA methylase
 
integrase Gene may contribute to site-specific recombination

ElementNo. of sequencesDownload
Nucleotide sequences1Fasta
Proteins27Fasta
(1) te Poele EM; Samborskyy M; Oliynyk M; Leadlay PF; Bolhuis H; Dijkhuizen L (2008). Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded. Plasmid. 59(3):202-16. [PudMed:18295883] in_silico
(2) te Poele EM; Habets MN; Tan GY; Ward AC; Goodfellow M; Bolhuis H; Dijkhuizen L (2007). Prevalence and distribution of nucleotide sequences typical for pMEA-like accessory genetic elements in the genus Amycolatopsis. FEMS Microbiol Ecol. 61(2):285-94. [PudMed:17535299] experimental
(3) Moretti P; Hintermann G; Hutter R (1985). Isolation and characterization of an extrachromosomal element from Nocardia mediterranei. Plasmid. 14(2):126-33. [PudMed:2999850] experimental
(4) Madon J; Moretti P; Hutter R (1987). Site-specific integration and excision of pMEA100 in Nocardia mediterranei. Mol Gen Genet. 209(2):257-64. [PudMed:2823074] experimental
 
experimental experimental literature
in_silico in silico analysis literature